Showing papers on "Volvariella volvacea published in 1989"

Preparation and Immunochemical Characterization of Antibody to Branched β-(1→3)-D-Glucan of Volvariella volvacea, and Its Use in Studies of Antitumor Actions

Abstract: Partially purified antibody specific to the antitumor, 0–6 branched β-(1→3)-d-glucan (WG), isolated from the cold alkali-extract of the fruiting body of Volvariella volvacea (Fukurotake) was obtained by immunization of rabbits with the conjugate of WG with bovine serum albumin (BSA). Hapten inhibition studies of the precipitation reaction of the antibody and the β-d-glucan with various (1→6)-linked and branched (1→3)-linked β-d-gluco-oligosaccharides showed that the antibody recognizes the sequence involving the non-reducing terminal glucosyl groups and possibly the branch points. The WG antibody also interacted with other branched β-(1→3)-d-glucans, but the reactivity differed depending on the degree of branching. In connection with the specificity of the antibody, the antibody to glucan polyalcohol (WG polyol), raised by immunization with WG polyol-BSA, recognized mainly the polyol groups in the side chains and a part of (1→ 3)-linked glucose residues in the main chain. In relation to the antitumor acti...