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Adam D. Silverman
Researcher at Northwestern University
Publications - 16
Citations - 761
Adam D. Silverman is an academic researcher from Northwestern University. The author has contributed to research in topics: Synthetic biology & Medicine. The author has an hindex of 7, co-authored 13 publications receiving 409 citations. Previous affiliations of Adam D. Silverman include University of Wisconsin-Madison.
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Cell-free gene expression: an expanded repertoire of applications.
TL;DR: Advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.
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Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor.
Walter Thavarajah,Adam D. Silverman,Matthew S. Verosloff,Nancy Kelley-Loughnane,Michael C. Jewett,Julius B. Lucks +5 more
TL;DR: Through onsite detection of fluoride in a real-world water source, this work provides a critical proof-of-principle for the future engineering of riboswitches and other biosensors to address challenges for global health and the environment.
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Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry
TL;DR: It is shown that cell-free expression of genes under bacterial σ70 promoters is constrained by the rate of transcription in crude extracts, and that processing the extract with a ribosomal runoff reaction and subsequent dialysis alleviates this constraint.
Journal ArticleDOI
Point-of-care biomarker quantification enabled by sample-specific calibration
Monica P. McNerney,Yan Zhang,Paige L. Steppe,Adam D. Silverman,Michael C. Jewett,Mark P. Styczynski +5 more
TL;DR: A platform for inexpensive, easy-to-use diagnostics that uses cell-free expression to generate colored readouts that are visible to the naked eye, yet quantitative and robust to the interference effects seen in complex samples is developed.
Journal ArticleDOI
Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid.
Xiangyang Liu,Adam D. Silverman,Khalid K. Alam,Erik Iverson,Julius B. Lucks,Michael C. Jewett,Srivatsan Raman,Srivatsan Raman +7 more
TL;DR: This work engineer a portable biosensor for cyanuric acid (CYA) using a LysR-type transcription regulator from Pseudomonas within the context of Escherichia coli gene expression machinery, and elucidates general principles to facilitate the engineering of a wider array of LTTR-based environmental sensors.