M
Michael C. Jewett
Researcher at Northwestern University
Publications - 269
Citations - 12822
Michael C. Jewett is an academic researcher from Northwestern University. The author has contributed to research in topics: Synthetic biology & Cell-free protein synthesis. The author has an hindex of 54, co-authored 230 publications receiving 9584 citations. Previous affiliations of Michael C. Jewett include Stanford University & Harvard University.
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Journal ArticleDOI
Cell-free protein synthesis: applications come of age.
TL;DR: In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
Journal ArticleDOI
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement.
Farren J. Isaacs,Peter A. Carr,Harris H. Wang,Marc J. Lajoie,Bram Sterling,Laurens Kraal,Andrew C. Tolonen,Tara A. Gianoulis,Daniel B. Goodman,Daniel B. Goodman,Nikos Reppas,Christopher J. Emig,Duhee Bang,Samuel J. Hwang,Michael C. Jewett,Michael C. Jewett,Joseph M. Jacobson,George M. Church +17 more
TL;DR: H hierarchical conjugative assembly genome engineering (CAGE) was developed to merge these sets of codon modifications into genomes with 80 precise changes, which demonstrate that these synonymous codon substitutions can be combined into higher-order strains without synthetic lethal effects.
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How many human proteoforms are there
Ruedi Aebersold,Jeffrey N. Agar,I. Jonathan Amster,Mark S. Baker,Carolyn R. Bertozzi,Emily S. Boja,Catherine E. Costello,Benjamin F. Cravatt,Catherine Fenselau,Benjamin A. Garcia,Ying Ge,Jeremy Gunawardena,Ronald C. Hendrickson,Paul J. Hergenrother,Christian G. Huber,Alexander R. Ivanov,Ole N. Jensen,Michael C. Jewett,Neil L. Kelleher,Laura L. Kiessling,Nevan J. Krogan,Martin R. Larsen,Joseph A. Loo,Rachel R. Ogorzalek Loo,Emma Lundberg,Emma Lundberg,Michael J. MacCoss,Parag Mallick,Vamsi K. Mootha,Milan Mrksich,Tom W. Muir,Steven M. Patrie,James J. Pesavento,Sharon J. Pitteri,Henry Rodriguez,Alan Saghatelian,Wendy Sandoval,Hartmut Schlüter,Salvatore Sechi,Sarah A. Slavoff,Lloyd M. Smith,Michael Snyder,Paul M. Thomas,Mathias Uhlén,Jennifer E. Van Eyk,Marc Vidal,David R. Walt,Forest M. White,Evan R. Williams,Therese Wohlschlager,Vicki H. Wysocki,Nathan A. Yates,Nicolas L. Young,Bing Zhang +53 more
TL;DR: This work frames central issues regarding determination of protein-level variation and PTMs, including some paradoxes present in the field today, and uses this framework to assess existing data and ask the question, "How many distinct primary structures of proteins (proteoforms) are created from the 20,300 human genes?"
Journal ArticleDOI
Mimicking the Escherichia coli cytoplasmic environment activates long‐lived and efficient cell‐free protein synthesis
TL;DR: By more closely replicating the physiological conditions of the cytoplasm of Escherichia coli, the Cytomim system provides a stable energy supply for protein expression without phosphate accumulation, pH change, exogenous enzyme addition, or the need for expensive high‐energy phosphate compounds.
Journal ArticleDOI
Cell-free synthetic biology: Thinking outside the cell
TL;DR: It is now clear that cell-free systems offer a versatile test-bed for understanding why nature's designs work the way they do and also for enabling biosynthetic routes to novel chemicals, sustainable fuels, and new classes of tunable materials.