Adrian Zander

TL;DR: Mutational analysis shows that key residues important for guide-dependent target processing are also involved in guide-independent MjAgo function, the first characterization of guide- independent cleavage activity for an Argonaute protein potentially serving as a guide biogenesis pathway in a prokaryotic system.

TL;DR: In insights into the molecular mechanisms that drive DNA-guided DNA silencing by an archaeal Ago, it is observed that the nature of the 3′ and 5′ nucleotides in particular, as well as the switch helix, appear to impact MjAgo cleavage activity.

TL;DR: Single-molecule FRET measurements demonstrate that the 3′end of the DNA guide is released from the PAZ domain upon target strand loading, which supports the two state model for Argonaute action.

TL;DR: This review focuses on fluorescence resonance energy transfer experiments and discusses how the optical single-molecule toolkit has been extended in recent years to capture the physiological complexity of a cell making it even more relevant for biological research.

TL;DR: This review discusses new findings in the field that shed light on the structure and function of Argonaute and especially focuses on archaeal Argonautes when discussing the details of the structural and dynamic features in ArgonaUTE that promote substrate recognition and cleavage, thereby revealing differences and similarities inArgonaute biology.