Adriano O. Henriques

TL;DR: New insights into the molecular details of spore surface morphogenesis provide avenues for exploitation of the sporesurface layers in applications for biotechnology and medicine.

TL;DR: This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3), and shows a half-life of inactivation at 80 °C of about 4 and 2 h, indicating that CotA is intrinsically highly thermostable.

TL;DR: The results that provided evidence of colonization, immunostimulation, and antimicrobial activity support the hypothesis that the organisms carried in five commercial probiotic products have a potential probiotic effect.

TL;DR: Together, the results suggest that some of the sporeformers isolated in this study have the potential to persist in or transiently associate with the complex gut ecosystem.

TL;DR: Comparisons with the structure of other monomeric multicopper oxidases containing four copper atoms suggest that CotA may accept the largest substrates of any known laccase, and unlike other laccases, CotA appears to have a flexible lidlike region close to the substrate-binding site that may mediate substrate accessibility.