Showing papers by "Ajit Varki published in 1988"

TL;DR: Improved methods to metabolically label cell lines with 35SO4 and to release sulfated N-linked oligosaccharides with peptide:N-glycosidase F are reported as well as the partial structure of some of these novel oligosACcharides.

TL;DR: An acetyl-coenzyme A: polysialosyl O-acetyltransferase activity that is found only in E. coli K1 OAc+ substrains is demonstrated, suggesting that enzymatic recognition of the substrate requires only thepolysialic acid sequence.

TL;DR: It is shown that bovine pulmonary arterial endothelial (CPAE) and human erythroleukemia (K562) cell lines also contain a class of more highly charged sulfated but less sialylated oligosaccharides, which suggests that the occurrence of these novel N-linked chains is not a random event resulting from accidental initiation of GAG chain synthesis on N- linked intermediates in the Golgi apparatus.

TL;DR: This study characterized a sulfophosphoglycoprotein of 140 kDa from FG-Met-2 pancreatic cancer cells whose oligosaccharides share some properties of both heparin and heparan-like sequences and the heterogeneity of these molecules is as extensive as that described for whole-cell sulfated N-linked oligosACcharides in the preceding two papers.