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Alan Tunnacliffe
Researcher at University of Cambridge
Publications - 96
Citations - 11064
Alan Tunnacliffe is an academic researcher from University of Cambridge. The author has contributed to research in topics: Desiccation tolerance & Cryptobiosis. The author has an hindex of 45, co-authored 96 publications receiving 10280 citations. Previous affiliations of Alan Tunnacliffe include University of Nottingham.
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Journal ArticleDOI
Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
Håkan Telenius,Nigel P. Carter,C. E. Bebb,M. Nordenskjold,Bruce A.J. Ponder,Alan Tunnacliffe +5 more
TL;DR: DOP-PCR represents a rapid, efficient, and species-independent technique for general DNA amplification, and has advantages over interspersed repetitive sequence PCR (IRS- PCR), which relies on the appropriate positioning of species-specific repeat elements.
Journal ArticleDOI
Trehalose, a Novel mTOR-independent Autophagy Enhancer, Accelerates the Clearance of Mutant Huntingtin and α-Synuclein
TL;DR: It is shown that trehalose also protects cells against subsequent pro-apoptotic insults via the mitochondrial pathway, which may be relevant to the treatment of HD and related diseases, where the mutant proteins are autophagy substrates.
Journal ArticleDOI
LEA proteins prevent protein aggregation due to water stress.
TL;DR: First evidence of anti-aggregation activity of LEA proteins due to water stress is found, which is significant given that non-reducing disaccharides are known to accumulate during dehydration in plants and nematodes.
Journal ArticleDOI
The continuing conundrum of the LEA proteins
Alan Tunnacliffe,Michael J. Wise +1 more
TL;DR: This review presents some new data, survey the biochemistry, biophysics and bioinformatics of the LEA proteins and highlights several possible functions, including roles as antioxidants and as membrane and protein stabilisers during water stress, either by direct interaction or by acting as molecular shields.
Journal ArticleDOI
Cytogenetic analysis by chromosome painting using dop-pcr amplified flow-sorted chromosomes
Håkan Telenius,A H Pelmear,Alan Tunnacliffe,Nigel P. Carter,A. Behmel,Malcolm A. Ferguson-Smith,Magnus Nordenskjöld,Roswitha Pfragner,Bruce A.J. Ponder +8 more
TL;DR: This study shows that flow sorting of aberrant chromosomes and chromosome painting can be used as a rapid aid to cytogenetic analysis, particularly in cases of difficult karyotypes, such as tumours.