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Alec A. K. Nielsen
Researcher at Massachusetts Institute of Technology
Publications - 18
Citations - 2748
Alec A. K. Nielsen is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Circuit design & Synthetic biology. The author has an hindex of 13, co-authored 18 publications receiving 2315 citations.
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Journal ArticleDOI
Genetic circuit design automation
Alec A. K. Nielsen,Bryan S. Der,Bryan S. Der,Jonghyeon Shin,Prashant Vaidyanathan,Vanya Paralanov,Elizabeth A. Strychalski,David J. Ross,Douglas Densmore,Christopher A. Voigt +9 more
TL;DR: Electronic design automation principles from EDA are applied to enable increased circuit complexity and to simplify the incorporation of synthetic gene regulation into genetic engineering projects, and it is demonstrated that engineering principles can be applied to identify and suppress errors that complicate the compositions of larger systems.
Journal ArticleDOI
Characterization of 582 natural and synthetic terminators and quantification of their design constraints
Ying-Ja Chen,Peng Liu,Alec A. K. Nielsen,Jennifer A N Brophy,Kevin Clancy,Todd Peterson,Christopher A. Voigt +6 more
TL;DR: The availability of many terminators of varying strength, as well as an understanding of the sequence dependence of their properties, will extend their usability in the forward design of synthetic cistrons.
Journal ArticleDOI
Genomic mining of prokaryotic repressors for orthogonal logic gates.
Brynne C. Stanton,Alec A. K. Nielsen,Alvin Tamsir,Kevin Clancy,Todd Peterson,Christopher A. Voigt +5 more
TL;DR: In this paper, the TetR-family repressor-promoter pairs were converted to a Not/NOR gate and characterized, and the operators of a subset were determined using an in vitro method, and this information was used to build synthetic promoters.
Genomic mining of prokaryotic repressors for orthogonal logic gates
Alvin Tamsir,Kevin Clancy,Todd Peterson,Brynne C. Stanton,Alec A. K. Nielsen,Christopher A. Voigt +5 more
TL;DR: “Part mining” is applied to build a library of 73 TetR-family repressors gleaned from prokaryotic genomes to build synthetic promoters that both strongly repress their cognate promoter and do not interact with other promoters.
Journal ArticleDOI
Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks
TL;DR: This work constructed a set of NOT gates by designing five synthetic Escherichia coli σ70 promoters that are repressed by corresponding sgRNAs, and these interactions do not exhibit crosstalk between each other.