Aleksandr Gaun

TL;DR: iFASP provides a quick, simple and effective method for obtaining clean samples, ensuring efficient digestion and providing excellent labeling yields for quantitative proteomics experiments, and compared the labeling yields and number of high-confidence peptide identifications to a standard in-solution digestion and labeling protocol.

TL;DR: A mass spectrometry–based tandem mass tag 9-plex strategy was used to determine alterations in relative protein abundance due to three carbon sources—glucose, galactose, and raffinose.

TL;DR: This study presents the first large-scale analysis of the proteomic and phosphoproteomic alterations in pancreatic stellate cells following treatment with two nicotinic acetylcholine receptor (nAChR) ligands: nicotine and α-bungarotoxin.

TL;DR: Subcellular fractionation of PaDC resulted in greater proteome coverage compared to PaDC whole cell lysate analysis, which allows for a greater depth of proteomic analysis and thus a better understanding of the cellular mechanisms of pancreatic disease.

TL;DR: In this paper, a fully automated multiplexed proteome profiling platform (AutoMP3) using the Hamilton Vantage liquid handling robot capable of preparing hundreds to thousands of samples was presented.