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Alexandre Juillerat
Researcher at École Polytechnique Fédérale de Lausanne
Publications - 13
Citations - 1546
Alexandre Juillerat is an academic researcher from École Polytechnique Fédérale de Lausanne. The author has contributed to research in topics: Fusion protein & Alkyltransferase. The author has an hindex of 8, co-authored 13 publications receiving 1406 citations. Previous affiliations of Alexandre Juillerat include École Normale Supérieure.
Papers
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Journal ArticleDOI
An Engineered Protein Tag for Multiprotein Labeling in Living Cells
Arnaud Gautier,Alexandre Juillerat,Christian Heinis,Ivan R. Corrêa,Maik Kindermann,Florent Beaufils,Kai Johnsson +6 more
TL;DR: The generation of an AGT-based tag is reported, named CLIP-tag, which reacts specifically with O2-benzylcytosine derivatives, which is derived from the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT).
Journal ArticleDOI
Directed Evolution of O6-Alkylguanine-DNA Alkyltransferase for Efficient Labeling of Fusion Proteins with Small Molecules In Vivo
Alexandre Juillerat,Thomas Gronemeyer,Antje Keppler,Susanne Gendreizig,Horst Pick,Horst Vogel,Kai Johnsson +6 more
TL;DR: The generation of mutants of the human O(6)-alkylguanine-DNA alkyltransferase (hAGT) for the efficient in vivo labeling of fusion proteins with synthetic reporter molecules and yield insight into the interaction of the DNA repair protein hAGT with its inhibitor O( 6)-benzylguAnine.
Journal ArticleDOI
Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling
TL;DR: The use of directed evolution and two different selection systems are reported to further increase the activity of AGT towards BG derivatives by a factor of 17 and demonstrate the advantages of this mutant for the specific labeling ofAGT fusion proteins displayed on the surface of mammalian cells.
Journal ArticleDOI
Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells
Alexandre Juillerat,Christian Heinis,India Sielaff,Jan Barnikow,Hugues Jaccard,Béatrice Kunz,Alexey V. Terskikh,Kai Johnsson +7 more
TL;DR: The synthesis of an inhibitor of wtAGT and the generation of AGT mutants that are resistant to this inhibitor are presented and this ability to specifically label AGT fusion proteins in the presence of endogenous AGT, after brief incubation of the cells with a small‐molecule inhibitor, is presented.
Patent
Labelling of Fusion Proteins With Synthetic Probes
TL;DR: In this article, a new proteins called alkylcytosine transferases (ACTs) derived from O6-alkylguanine-delta-alkyltransferase (AGT) are described.