An Engineered Protein Tag for Multiprotein Labeling in Living Cells
Arnaud Gautier,Alexandre Juillerat,Christian Heinis,Ivan R. Corrêa,Maik Kindermann,Florent Beaufils,Kai Johnsson +6 more
TLDR
The generation of an AGT-based tag is reported, named CLIP-tag, which reacts specifically with O2-benzylcytosine derivatives, which is derived from the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT).About:
This article is published in Chemistry & Biology.The article was published on 2008-02-22 and is currently open access. It has received 925 citations till now. The article focuses on the topics: SNAP-tag & Alkyltransferase.read more
Citations
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Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
TL;DR: The bioorthogonal chemical reactions developed to date are described and how they can be used to study biomolecules.
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Fluorescent probes for super-resolution imaging in living cells
TL;DR: The contributions of fluorescent probes to far-field super-resolution imaging, focusing on fluorescent proteins and organic small-molecule fluorophores are described, to reach the goal of video-rate imaging of live cells with molecular resolution.
Journal ArticleDOI
Functionalizing nanoparticles with biological molecules: developing chemistries that facilitate nanotechnology.
Kim E. Sapsford,W. Russ Algar,Lorenzo Berti,Kelly Boeneman Gemmill,Brendan J. Casey,Eunkeu Oh,Michael H. Stewart,Igor L. Medintz +7 more
TL;DR: Chemistries that Facilitate Nanotechnology Kim E. Sapsford,† W. Russ Algar, Lorenzo Berti, Kelly Boeneman Gemmill,‡ Brendan J. Casey,† Eunkeu Oh, Michael H. Stewart, and Igor L. Medintz .
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A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm,Brian P. English,Jiji Chen,Joel P Slaughter,Zhengjian Zhang,Andrey Revyakin,Ronak Patel,John J. Macklin,Davide Normanno,Robert H. Singer,Timothée Lionnet,Luke D. Lavis +11 more
TL;DR: Inspired by molecular modeling, the N,N-dimethylamino substituents in tetramethylrhodamine are replaced with four-membered azetidine rings, which doubles the quantum efficiency and improves the photon yield of the dye in applications ranging from in vitro single-molecule measurements to super-resolution imaging.
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Singlet oxygen: there is indeed something new under the sun
TL;DR: In this critical review, recent work on singlet oxygen is summarized, focusing primarily on systems that involve light.
References
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A guide to choosing fluorescent proteins.
TL;DR: A unified characterization of the best available FPs provides a useful guide in narrowing down the options for biological imaging tools.
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The fluorescent toolbox for assessing protein location and function
TL;DR: The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy.
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A general method for the covalent labeling of fusion proteins with small molecules in vivo
TL;DR: A general method for the covalent labeling of fusion proteins in vivo that complements existing methods for noncovalentlabeling of proteins and that may open up new ways of studying proteins in living cells is described.
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Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
TL;DR: This system provides a recipe for slightly modifying a target protein so that it can be singled out from the many other proteins inside live cells and fluorescently stained by small nonfluorescent dye molecules added from outside the cells.
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Multicolor and Electron Microscopic Imaging of Connexin Trafficking
Guido M. Gaietta,Thomas J. Deerinck,Stephen R. Adams,James C. Bouwer,Oded Tour,Dale W. Laird,Gina E. Sosinsky,Roger Y. Tsien,Mark H. Ellisman +8 more
TL;DR: This approach was used to show that newly synthesized connexin43 was transported predominantly in 100- to 150-nanometer vesicles to the plasma membrane and incorporated at the periphery of existing gap junctions, whereas older connexins were removed from the center of the plaques into pleiomorphic vesicle of widely varying sizes.
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