Alin Rai

TL;DR: A review of the biophysical properties and physiological functions of extracellular vesicles, particularly their pro-metastatic effects, and highlight the utility of EVs for the development of cancer diagnostics and therapeutics can be found in this paper.

TL;DR: The selective enrichment of metastatic factors and signaling pathway components in metastatic colon cancer cell‐derived exosomes contributes to the understanding of the cross‐talk between tumor and stromal cells in the tumor microenvironment.

TL;DR: A sequential centrifugal ultrafiltration (SCUF) method is developed and described here to examine, in an unbiased manner, what EV subtypes are released in vitro into cell culture medium using the human colon carcinoma cell line LIM1863 as a model system and reports for the first time that both SCUF-Exos andSCUF-sMVs isolate invasion of recipient NIH3T3 cells.

TL;DR: Surprisingly, miRNA passenger strands (star miRNAs) for miR-3613-3p* were the dominant strand in A33-Exos, the converse to that observed in parental cells, which suggests miRNA biogenesis may be interlinked with endosomal/exosomal processing.

TL;DR: This review focuses on the emerging importance of discovery proteomics and targeted proteomics (hypothesis-driven quantitative protein subset analysis) mass spectrometry (MS)-based strategies in EV biology, especially exosomes and shed microvesicles.