Showing papers by "Amanda Balish published in 2006"
TL;DR: MDCK cells are the preferred cell line for isolation of human influenza viruses from clinical specimens, and are divided into subtypes based on genetic and antigenic differences in the two surface spike proteins, hemagglutinin and neuraminidase.
Abstract: Influenza viruses are negative-sense, single-stranded, enveloped RNA viruses belonging to the family Orthomyxoviridae. Three types exist, influenza A, B, and C. All infect humans, but only A and B are major human pathogens. Influenza type A viruses are divided into subtypes based on genetic and antigenic differences in the two surface spike proteins, hemagglutinin (HA) and neuraminidase (NA). The appropriate cell lines to be used for isolation of influenza A or B viruses depend on the clinical information and the host of origin. MDCK cells are the preferred cell line for isolation of human influenza viruses from clinical specimens.
295 citations
TL;DR: The genetic evolution of avian influenza A(H5N1) viruses is accompanied during adaptation to poultry by the evolution of their receptor specificity, demonstrating them with an ability to bind to receptors optimal for human influenza viruses.
199 citations
TL;DR: This study underscores the importance of performing autopsies to identify the causes of death in patients with influenza infection and proves IHC assays are useful for the diagnosis of influenza and bacterial pneumonia.
Abstract: BACKGROUND The Centers for Disease Control and Prevention enhanced national surveillance for influenza-associated deaths among children because of early reports of pediatric deaths during the 2003-2004 influenza season. METHODS We studied lung and upper airway specimens from 47 case patients who died who had at least 1 positive result for influenza virus tests using hematoxylin and eosin, special stains for bacteria and fungi, and immunohistochemical (IHC) assays for influenza A and B viruses and other potential viral and bacterial respiratory pathogens. RESULTS Nineteen (40%) of the 47 patients were
127 citations