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Amine Kamen
Researcher at McGill University
Publications - 209
Citations - 9452
Amine Kamen is an academic researcher from McGill University. The author has contributed to research in topics: Cell culture & Virus. The author has an hindex of 53, co-authored 197 publications receiving 8578 citations. Previous affiliations of Amine Kamen include Nuclear Regulatory Commission & École Polytechnique de Montréal.
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High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
TL;DR: A scalable transfection procedure using polyethylenimine (PEI) is described for the human embryonic kidney 293 cell line grown in suspension and 10- and 3-fold increases in SEAP expression was obtained in 293E cells compared with pcDNA3.1 and pCEP4 vectors.
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Effect of surface charge on the cellular uptake and cytotoxicity of fluorescent labeled cellulose nanocrystals.
Khaled A. Mahmoud,Jimmy A. Mena,Keith B. Male,Sabahudin Hrapovic,Amine Kamen,John H. T. Luong +5 more
TL;DR: As the surface charge of CNC played an important role in cellular uptake and cytotoxicity, facile surface functionalization together with observed noncytotoxicity rendered modified CNC as a promising candidate for bioimaging and drug delivery systems.
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Large-scale transfection of mammalian cells for the fast production of recombinant protein.
TL;DR: This chapter aims to provide an overview of large-scale transfection technology with a particular emphasis on calcium phosphate and polyethylenimine-mediated gene transfer.
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Development and optimization of an adenovirus production process
Amine Kamen,Olivier Henry +1 more
TL;DR: The implementation of the findings at large scale led to the development of an optimized and robust integrated process for adenovirus production using HEK‐293 cells cultured in suspension and serum‐free medium.
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Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: peptone additives improve cell growth and transfection efficiency
Phuong Lan Pham,Sylvie Perret,Huyen Chau Doan,Brian Cass,Gilles St-Laurent,Amine Kamen,Yves Durocher +6 more
TL;DR: The use of the new serum‐free EBNA1 cell line with pept one‐enriched serum‐ free medium significantly improves protein expression compared to peptone‐less medium, and significantly increases their purification efficiency compared to serum‐containing medium.