BIOE 402. Medical Technology Assessment. 2 or 3 hours. Bioentrepreneur course. Assessment of medical technology in the context of commercialization. Objectives, competition, market share, funding, pricing, manufacturing, growth, and intellectual property; many issues unique to biomedical products. Course Information: 2 undergraduate hours. 3 graduate hours. Prerequisite(s): Junior standing or above and consent of the instructor.

“Bioinformatics” 특집을 내면서

「脆弱性(Vulnerability)」とは何か

The discovery that viruses may be the most abundant organisms in natural waters, surpassing the number of bacteria by an order of magnitude, has inspired a resurgence of interest in viruses in the aquatic environment. Surprisingly little was known of the interaction of viruses and their hosts in nature. In the decade since the reports of extraordinarily large virus populations were published, enumeration of viruses in aquatic environments has demonstrated that the virioplankton are dynamic components of the plankton, changing dramatically in number with geographical location and season. The evidence to date suggests that virioplankton communities are composed principally of bacteriophages and, to a lesser extent, eukaryotic algal viruses. The influence of viral infection and lysis on bacterial and phytoplankton host communities was measurable after new methods were developed and prior knowledge of bacteriophage biology was incorporated into concepts of parasite and host community interactions. The new methods have yielded data showing that viral infection can have a significant impact on bacteria and unicellular algae populations and supporting the hypothesis that viruses play a significant role in microbial food webs. Besides predation limiting bacteria and phytoplankton populations, the specific nature of virus-host interaction raises the intriguing possibility that viral infection influences the structure and diversity of aquatic microbial communities. Novel applications of molecular genetic techniques have provided good evidence that viral infection can significantly influence the composition and diversity of aquatic microbial communities.

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Virioplankton: viruses in aquatic ecosystems

Vibrios are ubiquitous and abundant in the aquatic environment. A high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals, fish, shrimp, sponges, squid, and zooplankton. Vibrios harbour a wealth of diverse genomes as revealed by different genomic techniques including amplified fragment length polymorphism, multilocus sequence typing, repetetive extragenic palindrome PCR, ribotyping, and whole-genome sequencing. The 74 species of this group are distributed among four different families, i.e., Enterovibrionaceae, Photobacteriaceae, Salinivibrionaceae, and Vibrionaceae. Two new genera, i.e., Enterovibrio norvegicus and Grimontia hollisae, and 20 novel species, i.e., Enterovibrio coralii, Photobacterium eurosenbergii, V. brasiliensis, V. chagasii, V. coralliillyticus, V. crassostreae, V. fortis, V. gallicus, V. hepatarius, V. hispanicus, V. kanaloaei, V. neonatus, V. neptunius, V. pomeroyi, V. pacinii, V. rotiferianus, V. superstes, V. tasmaniensis, V. ezurae, and V. xuii, have been described in the last few years. Comparative genome analyses have already revealed a variety of genomic events, including mutations, chromosomal rearrangements, loss of genes by decay or deletion, and gene acquisitions through duplication or horizontal transfer (e.g., in the acquisition of bacteriophages, pathogenicity islands, and super-integrons), that are probably important driving forces in the evolution and speciation of vibrios. Whole-genome sequencing and comparative genomics through the application of, e.g., microarrays will facilitate the investigation of the gene repertoire at the species level. Based on such new genomic information, the taxonomy and the species concept for vibrios will be reviewed in the next years.

Biodiversity of Vibrios

Antibiotic resistance continues to plague antimicrobial chemotherapy of infectious disease. And while true biocide resistance is as yet unrealized, in vitro and in vivo episodes of reduced biocide susceptibility are common and the history of antibiotic resistance should not be ignored in the development and use of biocidal agents. Efflux mechanisms of resistance, both drug specific and multidrug, are important determinants of intrinsic and/or acquired resistance to these antimicrobials, with some accommodating both antibiotics and biocides. This latter raises the spectre (as yet generally unrealized) of biocide selection of multiple antibiotic-resistant organisms. Multidrug efflux mechanisms are broadly conserved in bacteria, are almost invariably chromosome-encoded and their expression in many instances results from mutations in regulatory genes. In contrast, drug-specific efflux mechanisms are generally encoded by plasmids and/or other mobile genetic elements (transposons, integrons) that carry additional resistance genes, and so their ready acquisition is compounded by their association with multidrug resistance. While there is some support for the latter efflux systems arising from efflux determinants of self-protection in antibiotic-producing Streptomyces spp. and, thus, intended as drug exporters, increasingly, chromosomal multidrug efflux determinants, at least in Gram-negative bacteria, appear not to be intended as drug exporters but as exporters with, perhaps, a variety of other roles in bacterial cells. Still, given the clinical significance of multidrug (and drug-specific) exporters, efflux must be considered in formulating strategies/approaches to treating drug-resistant infections, both in the development of new agents, for example, less impacted by efflux and in targeting efflux directly with efflux inhibitors.

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Efflux-mediated antimicrobial resistance

Ocean upwelling and intense monsoonal activity based on late Miocene diatom assemblages from Neil Island, Andaman and Nicobar Islands, India

Coral bleaching a nemesis for the Andaman reefs: Building an improved conservation paradigm

The southwestern part of south Shillong plateau (Meghalaya, N-E India), designated as Sylhet Limestone Group is sub-divided into three lithounits i.e., Lakadong, Umlatdoh and Prang formations in ascending order. The Prang Formation is the youngest lithostratigraphic unit of the Sylhet Limestone Group and has been dated as Middle to early Upper Eocene based on the benthic foraminifera studies. Thin section analysis of carbonate rocks from Prang Formation, exposed in the Bholaganj limestone quarry yielded a rich assemblage of calcareous algae. The coralline algal assemblage comprises both non-geniculate and geniculate forms. The green algae are represented by species of Halimeda belonging to the family Halimedaceae. Palaeoecological interpretation based on diversity, growth-form analysis and taphonomic aspects of the algal assemblage indicate that in all probabilities the deposition of Prang Formation occurred in shallow, warm, shelf environment of normal salinity within the transgressive phase.

Palaeoecological Implications of Corallinacean Red Algae and Halimedacean Green Algae from the Prang Formation of South Shillong Plateau, Meghalaya

Till date the Lakadong, Umlatdoh and Prang Formations of Meghalaya have accounted for detailed taxonomic and palaeoecological investigations of numerous coralline red algal taxa. However, record of coralline red algae hitherto unknown from the overlying Kopili Formation (late Eocene). This is the first explicit record of corallinacean red algae from the foraminiferal limestones exposed in the lower part of Kopili Formation. The coralline algal assemblage in the presently studied facies is represented by four genera of non-geniculate taxa belonging to family Corallinaceae (mastophoroids) and family Hapalidiaceae (melobesioids). Thin-section studies pertaining to the growth-form analysis have shown that most of the corallines are encrusting with rare occurrences of protuberances and layered or foliose lamellae. Predominance of encrusting coralline algal forms is the characteristic feature of the present algal assemblage.

Amit Ghosh

Papers

Ocean upwelling and intense monsoonal activity based on late Miocene diatom assemblages from Neil Island, Andaman and Nicobar Islands, India

Coral bleaching a nemesis for the Andaman reefs: Building an improved conservation paradigm

Palaeoecological Implications of Corallinacean Red Algae and Halimedacean Green Algae from the Prang Formation of South Shillong Plateau, Meghalaya

First record of Coralline Red Algae from the Kopili Formation (late Eocene) of Meghalaya, N-E India

Podospermum gen. et sp. nov., an Acmopyle-like dispersed silicified ovule/seed from Lower Cretaceous intertrappean beds of the Rajmahal Basin, India