Amy M. Furda

TL;DR: A gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification of long DNA targets, which has been used extensively to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins.

TL;DR: The results establish a role for Lig3 in mitochondria, but distinguish it from its interacting protein Xrcc1, which can be circumvented by targeting Lig1 to the mitochondria or expressing Chlorella virus DNA ligase, the minimal eukaryal nick-sealing enzyme, or Escherichia coli LigA, an NAD-dependent ligase.

TL;DR: It is suggested that persistent mtDNA damage is not sufficient to cause mitochondrial dysfunction and levels of mitochondrial- and nuclear-encoded proteins using antibody analysis and a pharmacologic profile of mitochondria using the Seahorse Extracellular Flux Analyzer suggest this.

TL;DR: This chapter was written as a guide to using the long-amplicon quantitative PCR (QPCR) assay for the measurement of DNA damage in mammalian as well as nonmammalian species such as Caenorhabditis elegans, Drosophila melanogaster, and two species of fish.

TL;DR: The findings suggest that the E233G variant affects RAD51D functions and protein interactions and increases cellular chemoresistance, the first to analyze the functional effects of a clinically relevant RAD51d amino acid substitution.