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An Staes
Researcher at Ghent University
Publications - 85
Citations - 4745
An Staes is an academic researcher from Ghent University. The author has contributed to research in topics: Proteome & Quantitative proteomics. The author has an hindex of 35, co-authored 75 publications receiving 4104 citations. Previous affiliations of An Staes include Max Planck Society & Jewish General Hospital.
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Journal ArticleDOI
Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
Kris Gevaert,Marc Goethals,Lennart Martens,Jozef Van Damme,An Staes,Grégoire Thomas,Joël Vandekerckhove +6 more
TL;DR: A diagonal method to isolate N-terminal peptides from in vivo blocked proteins based on diagonal electrophoresis and diagonal chromatography is described.
Journal ArticleDOI
Chromatographic Isolation of Methionine-containing Peptides for Gel-free Proteome Analysis Identification Of More Than 800 Escherichia Coli Proteins
Kris Gevaert,Jozef Van Damme,Marc Goethals,Grégoire Thomas,Bart Hoorelbeke,Hans Demol,Lennart Martens,Magda Puype,An Staes,Joël Vandekerckhove +9 more
TL;DR: A novel gel-free proteomic technology was used to identify more than 800 proteins from 50 million Escherichia coli K12 cells in a single analysis, demonstrating the high dynamic range and the flexibility in the peptide sorting chemistry.
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Caspase-specific and nonspecific in vivo protein processing during Fas-induced apoptosis.
Petra Van Damme,Lennart Martens,Jozef Van Damme,Koen Hugelier,An Staes,Joël Vandekerckhove,Kris Gevaert +6 more
TL;DR: A comprehensive picture of protease substrates in anti-Fas–treated apoptotic human Jurkat T lymphocytes is generated and several new processing events by immunodetection and by an in vitro assay using recombinant caspases and synthetic peptides containing presumed cleavage sites are validated.
Journal ArticleDOI
Selecting protein N-terminal peptides by combined fractional diagonal chromatography
An Staes,Francis Impens,Francis Impens,Petra Van Damme,Petra Van Damme,Bart Ruttens,Bart Ruttens,Marc Goethals,Marc Goethals,Hans Demol,Hans Demol,Evy Timmerman,Evy Timmerman,Joël Vandekerckhove,Joël Vandekerckhove,Kris Gevaert,Kris Gevaert +16 more
TL;DR: The fully matured N-terminal COFRADIC protocol as it is currently routinely used is described, including the most substantial improvements that were made since its original publication.
Journal ArticleDOI
Identification and Functional Characterization of N-Terminally Acetylated Proteins in Drosophila melanogaster
Sandra Goetze,Ermir Qeli,Christian Mosimann,An Staes,An Staes,Bertran Gerrits,Bernd Roschitzki,Sonali Mohanty,Sonali Mohanty,Eva Niederer,Endre Laczko,Evy Timmerman,Evy Timmerman,Vinzenz Lange,Ernst Hafen,Ruedi Aebersold,Ruedi Aebersold,Ruedi Aebersold,Joël Vandekerckhove,Joël Vandekerckhove,Konrad Basler,Christian H. Ahrens,Kris Gevaert,Kris Gevaert,Erich Brunner +24 more
TL;DR: A new study reveals a functional rule for N-terminal acetylation in higher eukaryotes called the (X)PX rule and describes a generic method that prevents this modification to allow the study of N- terminal acetylations in any given protein.