The interaction of cells and bacteria with surfaces structured at the nanometre scale.

The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation

https://www.cell.com/trends/biotechnology/pdf/S0167-7799(09)00235-2.pdf

Classification of osseointegrated implant surfaces: materials, chemistry and topography

Surface characteristics of dental implants: A review.

Implant osseointegration and the role of microroughness and nanostructures: Lessons for spine implants

http://cmdmc.com.br/redecmdmc/lab/arquivos_publicacoes/3242_The%20effects%20of%20implant%20surface%20nanoscale%20features%20on%20osteoblastspecific.pdf

The effects of implant surface nanoscale features on osteoblast-specific gene expression.

Purpose The use of nanotechnology to enhance endosseous implant surfaces may improve the clinical control of interfacial osteoblast biology. This study investigated the influence of a nanostructure-coated implant surface on osteoblast differentiation and its effects on bone-to-implant contact (BIC) and removal torque values. Materials and methods Titanium disks were machined (M) or machined and subsequently treated by acid etching (Ac) or by dipping in an aluminum oxide solution (Al2O3). Surfaces were characterized by scanning electron microscopy, atomic force microscopy, and x-ray microanalysis. For the in vitro experiment, rat mesenchymal stem cells (rMSCs) were grown in osteogenic supplements on the disk surfaces for 3 days. Real-time polymerase chain reaction (PCR) was used to measure mRNA levels of several gene products (bone sialoprotein, osteocalcin, osteopontin, and RUNX-2). For the in vivo experiment, titanium implants were placed in rat tibiae and harvested after 3 to 21 days for measurement of bone-specific mRNA levels by real-time PCR. Removal torque and BIC were measured 3 to 56 days after placement. Results Average height deviation (Sa, in nm) values for M, Ac, and Al2O3 implants were 86.5, 388.4, and 61.2, respectively. Nanostructured Al2O3 topographic features applied to machined implants promoted MSC commitment to the osteoblast phenotype. Greater bone-specific gene expression was observed in tissues adjacent to Al2O3 implants, and associated increases in BIC and torque removal were noted. Conclusion Nanostructured alumina may directly influence cell behavior to enhance osseointegration.

Nanostructured alumina-coated implant surface: effect on osteoblast-related gene expression and bone-to-implant contact in vivo.

196 Rev Odontol Bras Central 2010;19(50) INTRODUCAO Nanotecnologia e a aplicacao de ciencia e engenharia em escala atomica. Esta tecnologia facilita a construcao de novos materiais e dispositivos pela manipulacao de atomos individuais e moleculas e permite a construcao atomo por atomo de minusculas estruturas (1–100nm), as quais tem novas propriedades e grandes aplicacoes nas ciencias da saude e biotecnologia. Embora muitas definicoes estejam ligadas ao termo “nanomaterial”, esta definicao esta relacionada aos materiais com topografia, em pelo menos uma dimensao, variando de 1 a 100nm (nanoestruturado), e de acordo com a forma pode ser dividido em solidos cristalinos com o tamanho dos graos variando de 1 a 100nm (nanocristais), camadas superficiais unicas ou multiplas com espessura de 1 a 10nm (nanorevestimentos), pos extremamente finos com particulas variando de 1 a 100nm (nanopos) e fibras tambem com diâmetro na faixa de 1 a 100nm (nanofibras). Nos implantes oseointegrados o desenvolvimento de superficies nano-estruturadas pode aumentar consideravelmente a adesao de celulas osseas e tambem a producao de matriz ossea necessaria no processo de mineralizacao e manutencao do osso que circunda o implante1-10. Ate o presente momento, embora se tenha conhecimento sobre as vantagens das superficies nanoestruturadas, poucos sistemas de implantes dentarios osseointegrados disponiveis comercialmente no mercado tem utilizado Efeito de Superficies de Implantes Nano-Estruturadas na Expressao de Genes de Osteoblastos e no Contato Osso-Implante In Vivo

/pdf/efeito-de-superficies-de-implantes-nano-estruturadas-na-34lbazhop7.pdf

Efeito de Superfícies de Implantes Nano-Estruturadas na Expressão de Genes de Osteoblastos e no Contato Osso-Implante In Vivo Effect of Nanostructured Implant Surfaces on Osteoblast Related Gene Expression and Bone-Implant Contact In Vivo

André Luiz Araújo

Papers

The effects of implant surface nanoscale features on osteoblast-specific gene expression.

Nanostructured alumina-coated implant surface: effect on osteoblast-related gene expression and bone-to-implant contact in vivo.

Efeito de Superfícies de Implantes Nano-Estruturadas na Expressão de Genes de Osteoblastos e no Contato Osso-Implante In Vivo Effect of Nanostructured Implant Surfaces on Osteoblast Related Gene Expression and Bone-Implant Contact In Vivo