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Andrea Alfieri
Researcher at University of Pavia
Publications - 14
Citations - 1050
Andrea Alfieri is an academic researcher from University of Pavia. The author has contributed to research in topics: Flavin group & Mixed Function Oxygenases. The author has an hindex of 10, co-authored 13 publications receiving 896 citations. Previous affiliations of Andrea Alfieri include University of Milan & Netherlands Cancer Institute.
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Journal ArticleDOI
Crystal structure of a Baeyer-Villiger monooxygenase.
TL;DR: The structural studies highlight the functional complexity of this class of flavoenzymes, which coordinate the binding of three substrates in proximity of the flavin cofactor with formation of two distinct catalytic intermediates.
Journal ArticleDOI
Multiple pathways guide oxygen diffusion into flavoenzyme active sites
Riccardo Baron,Conor T. Riley,Pirom Chenprakhon,Kittisak Thotsaporn,Remko T. Winter,Andrea Alfieri,Federico Forneris,Willem J. H. van Berkel,Pimchai Chaiyen,Marco W. Fraaije,Andrea Mattevi,J. Andrew McCammon +11 more
TL;DR: This study indicates that monooxygenase and oxidase flavoenzymes employ multiple funnel-shaped diffusion pathways to absorb O2 from the solvent and direct it to the reacting C4a atom of the flavin cofactor.
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Revealing the moonlighting role of NADP in the structure of a flavin-containing monooxygenase
TL;DR: Localization of mutations in human FMO3 that are known to cause trimethylaminuria (fish-odor syndrome) in the elucidated FMO structure provides a structural explanation for their biological effects.
Journal ArticleDOI
Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases.
Mark P.A. Luna-Vargas,Evangelos Christodoulou,Andrea Alfieri,Willem J. van Dijk,Magda Stadnik,Richard G. Hibbert,Danny D. Sahtoe,Marcello Clerici,Valeria De Marco,Dene R. Littler,Patrick H.N. Celie,Titia K. Sixma,Anastassis Perrakis +12 more
TL;DR: A collection of protein expression vectors for ligation-independent cloning, which allow co-expression strategies by implementing different affinity tags and antibiotic resistances, allowing for high-throughput screening for the expression of soluble proteins in a variety of vectors in E. coli and in insect cells.
Journal ArticleDOI
Structure of the monooxygenase component of a two-component flavoprotein monooxygenase.
Andrea Alfieri,Francesco Fersini,Nantidaporn Ruangchan,Methinee Prongjit,Pimchai Chaiyen,Andrea Mattevi +5 more
TL;DR: Comparative analysis with the structures of other flavoenzymes indicates that a distinctive feature of monooxygenases is the presence of specific cavities that encapsulate and stabilize the crucial hydroperoxyflavin intermediate.