Andrea De Luna

TL;DR: The results indicate that blood EVs are sufficient to induce chondrogenic gene expression changes in OA chondrocytes, while preventing proinflammatory cytokine release compared to full blood product.

TL;DR: Blood product preparation method, type of anticoagulant, differentiation time, and blood product concentration have a significant influence on stem cell viability and the differentiation potential, favouring no use of antICOagulation, shorter differentiation periods, and lower blood product concentrations.

TL;DR: An isolation protocol is developed for EVs from CPRP and hypACT that can also deplete lipoproteins, which are often co-isolated in EV research due to shared physical properties and to gain insights into the functional repertoire conveyed via EV-associated miRNAs.

TL;DR: The role of MSC-derived extracellular vesicles in in vitro and in vivo studies for cartilage regeneration will be summarized with special focus on transported miRNA which either favored the progression of OA or protected the cartilage from degradation.

TL;DR: In this paper, a co-culture system of primary OA chondrocytes and activated M1 macrophages was developed to model an OA joint in order to observe the effects of EVs in modulating the inflammatory environment.