Andrew Lugowski

TL;DR: Experimental and computational methods to determine transcriptome-wide RNA stabilities using both pharmacological inhibition of transcription and metabolic labeling are described and broader application of these approaches will further the understanding of mRNA decay and illuminate its contribution to different biological processes.

TL;DR: It is proposed that ME31B is a global repressor whose regulatory impact changes based on its biological context, likely as a consequence of translational repression in the context of robust mRNA decay.

TL;DR: These results indicate that posttranscriptional regulatory factors, such as microRNAs, influence the associations of PABP and other core factors, and do so without substantially affecting steady-state tail length.

TL;DR: This work presents DRUID (for determination of rates using intron dynamics), a new computational pipeline that is robust, easy to use, and freely available that will allow broad application of metabolic labeling for studies of transcript stability.

TL;DR: This work represents the most comprehensive study of signaling associated with egg activation to date, suggesting novel mechanisms that can be experimentally tested and providing a valuable resource for the broader research community.