Showing papers by "Anna Rita Migliaccio published in 2002"
TL;DR: Investigating the hemopoietic system during the aging of a GATA-1(low) colony showed that growth factor genes implicated in the development of myelofibrosis were all expressed in the marrow from the mutants at higher levels than in corresponding normal tissues.
212 citations
TL;DR: A novel reciprocal cross-talk between the two pathways appears to be mediated by the ER, and in all tested cells, activation of the signalling pathways has a proliferative role.
107 citations
TL;DR: Because NRBC dose predicted engraftment speed, inclusion of NRBCs in the T NC count does not reduce the effectiveness of the prefreezing TNC count as an index of the quality of a PCB unit as a graft.
57 citations
TL;DR: Results indicate that adult progenitor cells do not have a numerical advantage when transplanted into α-thalassemic fetuses, and are not detectable 2–3 weeks after the transplant.
Abstract: Our aim was to evaluate the number of progenitor cells circulating in an alpha-thalassemic fetus during its infusion in utero with paternal CD34(+) and adult red cells and to compare those values with those circulating in normal and non-thalassemic anemic fetuses of matched gestational age. The treatment of the alpha-thalassemic fetus has been described elsewhere. Fetal blood was obtained from normal and anemic fetuses by fetal blood sampling for diagnostic or therapeutic purposes according to a protocol approved by the human subject committee. The number of progenitor cells in fetal blood was estimated on the basis of the number of colonies they gave rise to in semisolid cultures. The alpha-thalassemic fetus, as did the other fetuses analyzed, contained high numbers (10(6)-10(7) depending on the age) of progenitor cells, values which were higher than the number (10(4)-10(5)) of paternal progenitor cells being transplanted. Progenitor cells with adult characteristics (adult kinetics of differentiation) were detected rapidly (10 min) after the CD34(+) cell infusion, but were not detectable 2-3 weeks after the transplant. These results indicate that adult progenitor cells do not have a numerical advantage when transplanted into alpha-thalassemic fetuses.
7 citations