A
Anne Silene Ponstein
Researcher at Syngenta
Publications - 14
Citations - 740
Anne Silene Ponstein is an academic researcher from Syngenta. The author has contributed to research in topics: Chitinase & Phytase. The author has an hindex of 9, co-authored 14 publications receiving 718 citations.
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Journal ArticleDOI
Only Specific Tobacco (Nicotiana tabacum) Chitinases and [beta]-1,3-Glucanases Exhibit Antifungal Activity.
Marianne Beatrix Sela-Buurlage,Anne Silene Ponstein,Sandra A. Bres-Vloemans,Leo Sjoerd Melchers,Peter J. M. van den Elzen,Ben J. C. Cornelissen +5 more
TL;DR: The extracellular washing fluid from leaves of transgenic plants expressing either of the secreted class I enzymes exhibited antifungal activity on F. solani germlings in vitro comparable to that of the purified vacuolar class I proteins.
Journal ArticleDOI
Isolation and characterisation of a class of carbohydrate oxidases from higher plants, with a role in active defence
Jerôme Hubertina Henricus Victor Custers,Stuart J. Harrison,Marianne Beatrix Sela-Buurlage,Els van Deventer,Wessel Lageweg,Peter W. A. Howe,Pieter J. van der Meijs,Anne Silene Ponstein,Bert Simons,Leo Sjoerd Melchers,Maarten Hendrik Stuiver +10 more
TL;DR: Extracts from salicylic acid-treated lettuce and sunflower leaves displayed very potent antimicrobial activity against a set of phytopathogens, and transgenic plants overexpressing Ha-CHOX displayed enhanced resistance to infection by this pathogen, and the resistance level was proportional to enzyme expression.
Journal ArticleDOI
Stable expression of Phytase (phyA) in canola (Brassica napus) seeds: towards a commercial product
Anne Silene Ponstein,Jacob B. Bade,Theo C. Verwoerd,Lucy Molendijk,Joost Storms,Rob F. Beudeker,Jan Pen +6 more
TL;DR: In the expressionrange studied, phytase expression was gene-dosage dependent and the accumulation ofphytase appeared to be fairly stable.
Patent
Plasmids for plant transformation and method for using the same
Maarten Hendrik Stuiver,Anne Silene Ponstein,Stephan Andreas Ohl,Goddijn Oscar Johannes Maria,Lambertus Henricus Simons,Bernardus Martinus Maria Dekker,Sietske Hoekstra,Hendrik Tigelaar,Nicolas Elzinga +8 more
TL;DR: In this article, the authors proposed an Agrobacterium mediated plant transformation, especially to transformation of plants with T-DNA, where read-through at the borders is prohibited, which can be done by inhibition of transfer of vector DNA to the plant cell by creating DNA binding sites outside the T-borders.
Patent
Antifungal proteins, dna coding therefore, and hosts incorporating same
Jerome Hubertus Henricus Victor Custers,Wessel Lageweg,Leo Sjoerd Melchers,Anne Silene Ponstein,Marianne Beatrix Sela-Buurlage,Maarten Hendrik Stuiver,Deventer-Troost Johanna Pieternella Els Van +6 more
TL;DR: In this article, an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, was presented.