Anne Wiersma

TL;DR: Domain homology analysis of the predicted 1,000-residue Msa protein identified known carbohydrate-binding domains, further supporting its role as a mannose adhesin that is likely to be involved in the interaction of L. plantarum with its host in the intestinal tract.

TL;DR: These results illustrate how a genome-scale metabolic model and associated constraint-based modeling techniques can be used to analyze the physiology of growth on a complex medium rather than a minimal salts medium.

TL;DR: The metabolic pathways of the lactic acid bacterium Lactobacillus plantarum WCFS1 were reconstructed on the basis of the annotated genome and the resulting pathway-genome database, LacplantCyc, was manually curated extensively.

TL;DR: Constrained-based elementary flux mode analysis was developed that predicted 3 out of 2669 possible flux modes to be optimal under the experimental conditions and explained lactate formation as the result of competition for oxygen by the other flux modes.

TL;DR: The capacity of Lactobacillus plantarum, a lactic acid bacterium found in many fermented food products and in the gastrointestinal tract of mammals, to produce Sorbitol from fructose-6-phosphate by reverting the sorbitol catabolic pathway in a mutant strain deficient for both l- and d-lactate dehydrogenase activities is investigated.