A
António Roldão
Researcher at Universidade Nova de Lisboa
Publications - 48
Citations - 1805
António Roldão is an academic researcher from Universidade Nova de Lisboa. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 17, co-authored 34 publications receiving 1475 citations. Previous affiliations of António Roldão include Chalmers University of Technology.
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Journal ArticleDOI
Virus-like particles in vaccine development
António Roldão,Maria Candida M. Mellado,Leda R. Castilho,Manuel J.T. Carrondo,Paula M. Alves +4 more
TL;DR: This article focuses on the essential role of VLP technology in new-generation vaccines against prevalent and emergent diseases and the implications of large-scale VLP production in the context of process control, monitorization and optimization.
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Large-scale production and purification of VLP-based vaccines.
TL;DR: The current choices and trends of large-scale production and purification of VLP-based vaccines generated through the baculovirus expression vector system using insect cells are reviewed.
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A systems-level approach for metabolic engineering of yeast cell factories
TL;DR: Examples on how systems and synthetic biology brought yeast metabolic engineering closer to industrial biotechnology are described in this review, and these examples should demonstrate the potential of a systems-level approach for fast and efficient generation of yeast cell factories.
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Triple layered rotavirus VLP production: kinetics of vector replication, mRNA stability and recombinant protein production.
Helena L. A. Vieira,Catarina Estêvão,António Roldão,Cristina Peixoto,Marcos F. Q. Sousa,Pedro E. Cruz,Manuel J.T. Carrondo,Paula M. Alves +7 more
TL;DR: This study highlights some of the reasons why the tricistronic baculovirus strategy is more efficient for production of triple layered rotavirus 2/6/7 VLP than monocistronic co-infection.
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Error assessment in recombinant baculovirus titration: evaluation of different methods.
TL;DR: Based on the cost, time and error analysis undertaken in this study, the end-point dilution assay, microculture tetrazolium assay and flow cytometric assay were found to be the techniques that combine all these three main factors better.