Showing papers in "Journal of Virological Methods in 2009"

TL;DR: Novel linear Ig heavy- and light-chain gene expression cassettes were designed to express Ig V(H) and V(L) genes isolated from sorted single B cells as IgG1 antibody without a cloning step to constitute a highly efficient strategy for rapid expression of Ig genes for high-throughput screening and analysis without cloning.

TL;DR: The SYBR Green I-based real-time detection system was used to establish a convenient one-step PERT assay (SG-PERT), which can be completed in 2h, is linear over six orders of magnitude and can be used to quantify retroviruses belonging to divergent species.

TL;DR: A new sensitive and automated chemiluminescent assay was developed for the quantitative determination of hepatitis C virus (HCV) core antigen (Ag) in human sera or plasma: the Abbott ARCHITECT HCV Ag test.

TL;DR: The data suggest that norovirus possesses greater thermostability than this commonly used surrogate and indicate that current food processing regimes for pasteurisation are insufficient to achieve inactivation of GII.4 NoVs.

TL;DR: The Seeplex Respiratory Virus Detection assay represents a significant improvement over the conventional methods for the detection of a broad spectrum of respiratory viruses.

TL;DR: The methods developed are easy to standardize and may be valuable tools for the control of viral contamination in source water and for assessing the efficiency of virus removal in drinking water treatment plants.

TL;DR: Simple production methods for high-titer lentiviral vector production involving transfection of HEK 293T cells immediately after plating were developed and high titers were obtained with cell culture media lacking serum or other protein additives altogether, resulting in more consistent lentviral vector stocks.

TL;DR: The results of this study demonstrate that the real-time RT-PCR assay for rotavirus is broadly reactive, specific, and sensitive for detection of rotaviruses in clinical specimens and water samples.

TL;DR: It is suggested that detection and recovery of each enteric virus with this adsorption-elution method requires a specific MgCl(2) concentration and depends on the source of environmental water tested.

TL;DR: Qualitative PCR assays targeting specifically porcine adenoviruses have been developed and applied to fecal and environmental samples, including pig slurries, urban sewage, slaughterhouse sewage and river water samples and support the detection by real-time quantitative PCR as a promising and valuable tool for source-tracking studies.

TL;DR: A comparison of results obtained from 67 clinical samples using existing RT-PCR, shell vial, cell culture and ELISA methods showed that QCM techniques were comparable in sensitivity and specificity to cell culture methods.

TL;DR: The potential for the LFD to be used next to the animal in the pen-side diagnosis of FMD and for providing rapid and objective support to veterinarians in their clinical judgment of the disease is illustrated.

TL;DR: The sensitivity of the LAMP assay was determined to be a single copy of the standard plasmid, which was 100 fold and 10 fold higher than PCR and nested PCR, respectively; furthermore, no cross-reactivity was founded with the other tested viruses.

TL;DR: Based on the cost, time and error analysis undertaken in this study, the end-point dilution assay, microculture tetrazolium assay and flow cytometric assay were found to be the techniques that combine all these three main factors better.

TL;DR: A reverse transcription loop-mediated isothermal amplification method for the detection of peach latent mosaic viroid (PLMVd) was developed and was more sensitive than RT-PCR, and was detected in peach, plum, apricot, pear, wild pear and quince samples.

TL;DR: DNA copy numbers were more abundant in MS patients than in healthy humans, while syncytin-1 were unchanged, reinforcing the link between MSRV and MS.

TL;DR: A rapid and sensitive method by using a filtration concentration method followed by PCR amplification for detection of NoVs from cheese and fresh lettuce is developed and will help establish the cause and source of NoV outbreaks of food-borne illness.

TL;DR: A lateral-flow based assay using super-paramagnetic particles as the detection marker was developed for the detection of HIV-1 p24 antigen and could provide a simple, low-cost, and portable method for rapid HIV- 1 p24 detection in a variety of testing environments.

TL;DR: The results suggest that the combination of the multiplex PCR and multiplex RT-PCR is useful for rapid and accurate identification of nine major pathogenic viruses in pigs with multiple infections.

TL;DR: Analysis of competition between the individual reactions within the multiplex real-time PCR assay showed that GI and GII NoV plasmid DNAs mixed at equimolar concentrations were detected reproducibly and quantitatively, while a 4 log excess between GI andGII plasmID DNAs hindered amplification of the target with the lowest concentration.

TL;DR: The RT-LAMP-LFD method confirmed amplicon identity by hybridization and eliminated the need to handle carcinogenic ethidium bromide and the sensitivity of detection was comparable to that of other commonly used methods for nested RT-PCR detection of IMNV.

TL;DR: The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial d DengueNS1 antigen-capture ELISA.

TL;DR: The aim of this work was to develop a VIGS vector based on the Grapevine virus A (GVA), which is a member of the genus Vitivirus, family Flexiviridae, which may constitute an important tool for improving functional genomics in V. vinifera.

TL;DR: Results show that DBS can be used accurately instead of whole blood for the diagnosis of HIV-1 infection and VL quantitation, particularly if samples are appropriately stored, and could be consistently determined when stored frozen.

TL;DR: The PapilloCheck test and the Linear Array test give comparable results for detecting HPV in cervical specimens, however, these results suggest that there is a need to standardize the type-specific sensitivity of genotyping methods and to evaluate their accuracy to detect multiple HPV infections.

TL;DR: This study provides observations on the use of virus concentration methods for the detection of uncultivable HuNoV in water samples and suggests that both Mg- and Al-methods can be appropriate for concentrating HuNoVs from water samples.

TL;DR: Compared to previous methods for localizing virus transcripts in plant and insect tissues that include complex steps for in-vitro probe preparation or antibody raising, tissue fixation, block preparation, sectioning and hybridization, the method described below provides very reliable, convincing, background-free results with much less time, effort and cost.

TL;DR: Three different pathogenic CPV variants circulating currently in the Argentine domestic dog population were identified, with CPV2c being the only variant affecting vaccinated and unvaccinated dogs during the year 2008.

TL;DR: Nasal washes yielded the highest rate of viral detection without excessive patient discomfort, and nasal brushes produced the lowest detection rates and demonstrated the highest level of discomfort.

TL;DR: A neutralization assay using VSV pseudotype particles expressing the F and G proteins of NiV (pVSV-NiV-F/G) as target antigens has the potential to be a rapid and cost-effective diagnostic method, especially in locations that lack high containment facilities, and will provide a valuable tool for basic research and vaccine development.