Aurélie Claudine Periat

TL;DR: This work shows that HILIC can be successfully employed for the analysis of therapeutic proteins and mAbs, using mobile phase compositions comprised of between 65 and 80% ACN and 0.1% TFA, showing orthogonality to RPLC and improved separations of glycoforms.

TL;DR: Screening four different columns packed with sub-2μm particles at two mobile phase pH, using a fast gradient seems to be a good generic approach for initial HILIC method development.

TL;DR: The results prove that the ionic character of analytes in solution and the ionization mechanism (i.e., proton transfer) also play an important role for explaining the sensitivity enhancement in HILIC.

TL;DR: This review summarizes the recent advances in the analysis of amino acids, peptides, and proteins using hydrophilic interaction chromatography and expects its use for peptide mapping will continue to grow in the future, particularly because this analytical strategy can be combined with reversed-phase liquid chromatography, in a two-dimensional setup, to reach very high resolving power.

TL;DR: The recently proposed predictive elution window shifting and stretching (PEWS(2)) approach can be used, where only an (approximate) prediction of the retention of the first and the last peak in the chromatogram is required to conduct a well-targeted trial-and-error search.