Ayu Rahmawati Sulistyaningtyas

Abstract: Robusta is the most widely cultivated coffee in Indonesia (90% of the total Indonesian coffee production) Antioxidant activity and polyphenol content of Robusta coffee is higher than Arabica coffee or the other plants One of the efforts, for coffee market expansion is product diversification through decaffeination of coffee Decaffeination is one of process to reduce caffeine content Decaffeinated coffee (decaff coffee) began to become public demand In addition to its more delicious taste, low caffeine coffee is beneficial for health because it is safer to eat Coffee decaffeination can utilize organic solvents that contain proteases Tofu waste was one of protease sources So that, tofu waste had potential as solvent in decaffeination The objective of the study was to assess the antioxidant activity of Robusta green coffee after going through decaffeination by using tofu waste The study was used Factorial Completely Randomized Design, ie concentration of tofu waste (30%,60%, 90%) and length of immersion (3, 6, 9 hours) Robusta green coffee antioxidant activity was examined by using DPPH method The results showed that the highest antioxidant activity ( 176061) was in the treatment of 90% waste concentration and 9 hours of decaffeination time In conclusion, the greater the concentration and the longer the decaffeination resulted in the higher antioxidant activity This study provides information about coffee processing methods that can produce coffee with the best quality So, It can increasing the value of domestic coffee products especially in the global market and utilizing tofu liquid waste to be more malleable

Abstract: Lupus or Systemic Lupus Erythematosus (SLE) is a life-threatening chronic autoimmune disease with symptoms similar to those of other diseases. Frequent misdiagnosis of lupus increases the mortality rate of the disease. Public understanding is still low about Lupus, characterized by a variety of misperceptions about the disease, which still occurs. In rural area, Lupus is still often regarded as a virus transmitted and is associated with weak immune activity. There are still many women who do not yet know that they are a group that is more susceptible to Lupus than men. Various errors in people's perception of Lupus need to be corrected to increase public awareness of the disease. Efforts to correct misperceptions about Lupus have been done through Science and Technology for Community (Ilmu Pengetahuan dan Teknologi bagi Masyarakat, IbM) activities in counseling form to the housewife groups in Kalipucung Hamlet, Genting Village, Jambu District, Ambarawa, Central Java. Participatory approaches and tutorials have been carried out in the IbM activities through the provision of counseling materials, as well as the delivery of questionnaires before and after counseling. The results of the questionnaire analysis indicate that the counseling program could improve awareness about Lupus disease among community in rural area.

Abstract: Industri tempe merupakan industri skala rumah tangga yang jumlahnya hampir tersebar diseluruh wilayah Indonesia. Sebagian besar industri tempe tersebut masih dikelola secara sederhana. Terutama dalam hal pengolahan limbah. Penggunaan bakteri indigenous yang mampu mendegradasi senyawa organik dalam limbah bisa menjadi salah satu upaya untuk menangani kasus pencemaran lingkungan perairan dengan upaya bioremediasi. Penelitian ini bertujuan untuk mengisolasi bakteri indigenous penghasil enzim protease untuk digunakan sebagai agen bioremediasi limbah cair industri tempe. Isolasi sampel limbah cair industri tempe yang diambil dari Desa Sambirejo, Gayamsari, Kota Semarang dimurnikan dengan menggunakan media Nutrient broth (NB), kemudian diinokulasi dan dipurifikasi menggunakan media Nutrient Agar (NA). Koloni yang sudah dimurnikan kemudian dilakukan uji kemampuan bakteri dalam memfermentasi laktosa, menggunakan media Mac Conkey Agar (MC), dan uji kemampuan bakteri dalam menghemolisa sel darah merah menggunakan media Blood Agar Plate (BAP). Uji aktivitas hidrolitik dilakukan menggunakan media selektif yaitu Skim Milk Agar (SMA). Dari hasil penelitian yang sudah dilakukan, diperoleh 3 isolat yang berbeda yaitu isolat P2, P3.dan P4. Pada uji MC isolat P2 bersifat non laktosa fermenter sedangkan isolat P3 dan P4 bersifat laktosa fermenter. Setelah dilakukan uji BAP didapatkan hasil isolat P2, P3, dan P4 bersifat γ hemolisis. Pada uji aktivitas hidrolitik menggunakan media selektif, diperoleh 2 isolat mampu menghasilkan enzim protease yaitu isolat P2 dan P4. Kesimpulannya, Isolat P4 merupakan isolat yang berpotensi sebagai agen bioremediasi karena mampu menghasilkan zona bening dan tingkat patogenitas rendah. Kata kunci: Bakteri, bioremediasi, limbah, protease, tempe

Abstract: Orange sweet potato (OSP) and red rice (RR) are rich sources of health benefit-associated substances and can be conventionally cooked or developed into food products. This research approach was to closely monitor the changes of bioactive compounds and their ability as antioxidants from the native form to the food products which are ready to be consumed. Moreover, this research explored the individual carotenoids and tocopherols of raw and cooked OSP and RR and their developed flake products, and also investigated their antioxidant activity, physicochemical properties, and sensory properties. Simultaneous identification using the liquid chromatographic method showed that OSP, RR, and their flake products have significant amounts (µg/g) of β-carotene (278.58–48.83), α-carotene (19.57–15.66), β-cryptoxanthin (4.83–2.97), α-tocopherol (57.65–18.31), and also γ-tocopherol (40.11–12.15). Different responses were observed on the bioactive compound and antioxidant activity affected by heating process. Meanwhile, OSP and RR can be combined to form promising flake products, as shown from the physicochemical analysis such as moisture (5.71–4.25%) and dietary fiber (13.86–9.47%) contents, water absorption index (1.69–1.06), fracturability (8.48–2.27), crispness (3.9–1.5), and color. Those quality parameters were affected by the proportions of OSP and RR in the flake products. Moreover, the preference scores (n = 120 panelists) for the flakes ranged from slightly liked to indifferent. It can be concluded that OSP and RR are potential sources of bioactive compounds which could act as antioxidants and could be developed into flake products that meet the dietary and sensory needs of consumers.

Abstract: The use of yeasts as starter cultures was boosted with the emergence of large-scale fermentations in the 20th century. Since then, Saccharomyces cerevisiae has been the most common and widely used microorganism in the food industry. However, Candida species have also been used as an adjuvant in cheese production or as starters for coffee, cocoa, vegetable, meat, beer, and wine fermentations. A thorough screening of candidate Candida is sometimes performed to obtain the best performing strains to enhance specific features. Some commonly selected species include C. pulcherrima (teleomorph Metschnikowia pulcherrima) (wine), C. parapsilosis (teleomorph Monilia parapsilosis) (coffee), C. famata (teleomorph Debaryomyces hansenii) (cheese), and C. zeylanoides (teleomorph Kurtzmaniella zeylanoides) and C. norvegensis (teleomorph Pichia norvegensis) (cocoa). These species are associated with the production of key metabolites (food aroma formation) and different enzymes. However, safety-associated selection criteria are often neglected. It is widely known that some Candida species are opportunistic human pathogens, with important clinical relevance. Here, the physiology and metabolism of Candida species are addressed, initially emphasizing their clinical aspects and potential pathogenicity. Then, Candida species used in food fermentations and their functional roles are reported. We recommended that Candida not be used as food cultures if safety assessments are not performed. Some safety features are highlighted to help researchers choose methods and selection criteria.

Abstract: Excessive population outbursts and associated xenobiotic interventions contribute overproduction of waste materials across the world. Among these waste materials biomedical wastes (BMW) make a significant contribution. The huge accumulation of BMW is not only meant for successive environmental pollution but increases health hazards by cross-contamination and reoccurrence of different fatal infections. The management of BMW gaining continuous attention to the scientific communities for their intriguing potentiality towards public health concerns. Although, world health organization (WHO) and other public health and environmental societies formulate different guidelines for the disposal machinery of BMW but the proper implementation of those rules in public sectors in developing countries is very difficult. In this situation, the sudden prevalence of pandemic like, COVID-19 further worsen such conditions. Huge disposition of medical wastes during COVID-19 detection, treatment, and precautionary measures not only increases the risk of reoccurrence of infection but puts us also in front of a huge challenge of efficient management of these BMW. In this respect, the present review focus on an overview of BMW, existing BMW management, probable consequences of COVID-19 pandemic on the waste management system, and future perspectives.

Abstract: Robusta is the most widely cultivated coffee in Indonesia (90% of the total Indonesian coffee production) Antioxidant activity and polyphenol content of Robusta coffee is higher than Arabica coffee or the other plants One of the efforts, for coffee market expansion is product diversification through decaffeination of coffee Decaffeination is one of process to reduce caffeine content Decaffeinated coffee (decaff coffee) began to become public demand In addition to its more delicious taste, low caffeine coffee is beneficial for health because it is safer to eat Coffee decaffeination can utilize organic solvents that contain proteases Tofu waste was one of protease sources So that, tofu waste had potential as solvent in decaffeination The objective of the study was to assess the antioxidant activity of Robusta green coffee after going through decaffeination by using tofu waste The study was used Factorial Completely Randomized Design, ie concentration of tofu waste (30%,60%, 90%) and length of immersion (3, 6, 9 hours) Robusta green coffee antioxidant activity was examined by using DPPH method The results showed that the highest antioxidant activity ( 176061) was in the treatment of 90% waste concentration and 9 hours of decaffeination time In conclusion, the greater the concentration and the longer the decaffeination resulted in the higher antioxidant activity This study provides information about coffee processing methods that can produce coffee with the best quality So, It can increasing the value of domestic coffee products especially in the global market and utilizing tofu liquid waste to be more malleable

Abstract: Excessive population outbursts and associated xenobiotic interventions contribute overproduction of waste materials across the world. Among these waste materials biomedical wastes (BMW) make a significant contribution. The huge accumulation of BMW is not only meant for successive environmental pollution but increases health hazards by cross-contamination and reoccurrence of different fatal infections. The management of BMW gaining continuous attention to the scientific communities for their intriguing potentiality towards public health concerns. Although, world health organization (WHO) and other public health and environmental societies formulate different guidelines for the disposal machinery of BMW but the proper implementation of those rules in public sectors in developing countries is very difficult. In this situation, the sudden prevalence of pandemic like, COVID-19 further worsen such conditions. Huge disposition of medical wastes during COVID-19 detection, treatment, and precautionary measures not only increases the risk of reoccurrence of infection but puts us also in front of a huge challenge of efficient management of these BMW. In this respect, the present review focus on an overview of BMW, existing BMW management, probable consequences of COVID-19 pandemic on the waste management system, and future perspectives.