Bastian Minkenberg

TL;DR: A general strategy to produce numerous gRNAs from a single polycistronic gene via the endogenous tRNA-processing system is developed and shown to significantly increase CRISPR/Cas9 multiplex editing capability and efficiency in plants and is expected to have broad applications for small RNA expression and genome engineering.

TL;DR: A new resistance locus is described that encodes a non-NLR Armadillo repeat protein required for broad-spectrum resistance in rice and may aid in the development of broad spectrum blast resistant rice.

TL;DR: The CRISPR/Cas9 system is a prevalent and versatile genome-editing tool of choice for basic and applied biological research and is expected to accelerate functional discovery of plant genes as well as genetic improvement of agricultural crops.

TL;DR: Rice MAP kinase genes are used to illustrate the utility of a CRISPR/Cas9‐based genome editing strategy to genetically characterize and identify essential or lethal genes polycistronic tRNA‐gRNA gene editing allows efficient generation of a diversity of allelic mutations at multiple genomic sites and facilitates the functional discovery of redundant and/or essential genes.

TL;DR: Off-targets are sequences at other 5'-NGG-3' protospacer adjacent motifs (PAMs) that are identical or have a small number of mismatches or substitutions compared to the gRNA spacer or the targeted DNA.