Showing papers by "Bernhard Tribukait published in 1989"
TL;DR: It is concluded that the process of evolution from malignantly transformed lesions, confined to the Urothelium, to an exophytic or invasive tumor is dependent on a further elevated proliferation of the urothelial lesions.
27 citations
TL;DR: Although patients with stable disease had a significantly larger proportion of tumors with diploid DNA content, all except four patients were dead within a median survival period of 12 months; however, a significantly shorter survival time was noted in patients whose ascites comprised cell populations with S-phase values exceeding 15%.
20 citations
TL;DR: It is indicated that DNA analysis may be useful for defining the malignant potential of urothelial tumors of the upper urinary tract more fully than conventional grading and staging permit.
Abstract: A prospective study comprised operative specimens from 11 patients with transitional cell carcinoma of the renal pelvis or ureter. DNA analysis of the primary tumor and of multiple biopsy specimens from preselected sites of the surrounding urothelium was performed with flow cytometry. All Grade 3 tumors and 50% of the Grade 2 tumors were aneuploid, and the remainder were Grade 2 and diploid. All invasive tumors were aneuploid. Carcinoma in situ was found in some of the preselected biopsy specimens, all of which were aneuploid, from two patients. Close correlation thus was observed between aneuploidy and tumor invasiveness, whereas diploidy was seen only in noninvasive tumors with lower malignancy grade. Aneuploidy was also associated with increased risk of carcinoma in situ. The study indicated that DNA analysis may be useful for defining the malignant potential of urothelial tumors of the upper urinary tract more fully than conventional grading and staging permit. Cancer 64:2141-2145, 1989. ROGRESS IN THE ENDOSCOPIC MANAGEMENT Of UpP per urinary tract tumors emphasizes the need for more precise preoperative evaluation of such tumors than is possible by staging and grading. DNA analysis by flow cytometry (FCM) is a relatively new and promising technique for evaluating urothelial tumors of the urinary bladder, but its potential is less clear regarding tumors of the renal pelvis and ureter. The current study, therefore, compares the results of DNA analysis by FCM with the histopathologic features in carcinoma of the renal pelvis and ureter and evaluates the extent to which such analysis may supply information additional to the histologic data.
19 citations
TL;DR: Flow cytometry was performed in 82 paraffin-embedded clinical stage I cutaneous melanomas and a significant correlation of ploidy to melanoma thickness was found, but using the Cox’s proportional hazard test the influence of ploidsy vanished and melanoma Thickness or Clark level of invasion together with sex and location were the factors most important for prognosis.
Abstract: In a retrospective study flow cytometry was performed in 82 paraffin-embedded clinical stage I cutaneous melanomas. A significant correlation of ploidy to melanoma thickness was found. Using a log rank test ploidy was found significant for prognosis. However, using the Cox's proportional hazard test the influence of ploidy vanished and melanoma thickness or Clark level of invasion together with sex and location were the factors most important for prognosis.
18 citations
TL;DR: DNA-aneuploidy seems to appear earlier than dysplasia and may be an early marker of malignant transformation of the mucosa in ulcerative colitis, and Prospective trials are needed before the clinical significance of early findings of aneuploids can be determined.
Abstract: In seven patients with longstanding ulcerative colitis, multiple mucosal biopsies taken at colonoscopy were assessed for DNA content and histological dysplasia. DNA analyses were performed using flow cytometric analyses in biopsies and microspectrophotometry in imprint slides prepared from the biopsies. Abnormal aneuploid DNA content was detected in five of the patients in eight separate locations in the colon. There was good conformity between the two methods in the detection of aneuploidy. Both methods of DNA analysis are considered to be applicable in mucosal biopsies from patients with ulcerative colitis. In two patients with significant low grade dysplasia in association with macroscopical lesions (DALMs), there was a relationship with aneuploid DNA pattern found by flow cytometric or microspectrophotometric analyses. Indefinite changes, probably dysplastic were found in three other patients and two of those also displayed aneuploidy, although only once in the same biopsy location. In five locations aneuploidy was detected without concomitant dysplasia. DNA-aneuploidy seems therefore to appear earlier than dysplasia and may be an early marker of malignant transformation of the mucosa in ulcerative colitis. Prospective trials are needed before the clinical significance of early findings of aneuploidy can be determined.
17 citations
TL;DR: Bromodeoxyuridine (Brd-Urd) was utilized in vitro to detect proliferating cells in 33 samples of ascitic and pleural effusions of ovarian malignancies and the results were compared with DNA flow-cytometry.
Abstract: Bromodeoxyuridine (Brd-Urd) was utilized in vitro to detect proliferating cells in 33 samples of ascitic and pleural effusions of ovarian malignancies and the results were compared with DNA flow-cytometry. Percoll separated tumor cells were incubated in vitro with Brd-Urd. After treatment with anti-Brd-Urd monoclonal antibodies the proportion of fluorescent cells was evaluated on slides by microscopy. A good correspondence (r=0.93) was found between the proportion of cells labelled with Brd-Urd and the S-phase cells evaluated from the DNA histograms. S-phase values were found to be higher in tumors with aneuploid than in diploid DNA index. Poorly differentiated tumors had higher values as compared to moderately or well differentiated tumors.
12 citations
TL;DR: Chromosomal evolution could be studied in one case, in which both the primary tumor (T9) and its xenograft passages (1, 4, and 7), obtained 1, 7, and 11 months after the first transplantation of T9 cells to nude mice, were analyzed.
10 citations
TL;DR: The antitumor effect of human natural and recombinant interferon-γ (IFN-γ) was evaluated in human osteosarcomas grown as xenografts in nude mice.
Abstract: The antitumor effect of human natural and recombinant interferon-γ (IFN-γ) was evaluated in human osteosarcomas grown as xenografts in nude mice. IFN-γ was given as daily subcutaneous inje...
9 citations
TL;DR: It is concluded that moderate increases in temperature alone, or in combination with ADR, inhibit salvage DNA synthesis by inhibition of TdR uptake, possibly due to inhibition of phosphorylation;ADR, on the contrary, inhibits TDR uptake by other mechanisms such as changes in membrane function.
Abstract: Inhibition of the salvage DNA synthesis was studied in ascites tumour cells up to 240 min at moderately increased temperatures of 39°C, 40.5°C and 43°C alone or in combination with adriamycin (ADR). Hyperthermia and ADR acted additively on salvage DNA synthesis. The [3H]thymidine (TdR) incorporation was strongly related to the cellular uptake of [3H]TdR. In order to evaluate the significance of the decrease in [3H]TdR uptake for the incorporation into DNA, the thymidine kinase (TK) has also been studied. TK activity started to decrease at a temperature of 39°C, probably due to inhibition of translation and/or transcription of the enzyme, and was almost completely inhibited at 43°C. ADR did not affect TK activity. Inhibition of DNA synthesis by aphidicolin neither decreased the ability of the cell to take up [3H]TdR nor the ability to affect TK activity. We concluded that moderate increases in temperature alone, or in combination with ADR, inhibit salvage DNA synthesis by inhibition of TdR uptake, possibly...
8 citations
Journal Article•
TL;DR: The results indicate that the hormonal status and/or age are of importance for the development of genetically different forms of endometrial carcinomas, as judged from the DNA-distribution.
Abstract: In a prospective study of 283 women with endometrial carcinoma, 29 of whom were premenopausal and 254 postmenopausal, the DNA content and S-phase rate were measured by flow cytometry. Over 90% of the tumors in premenopausal women were peri-diploid and the remaining tumors were tetraploid, whereas 23% of the tumors in post-menopausal women were grossly aneuploid (p less than 0.01). Similar differences were found between women younger or older than 50 years. The results indicate that the hormonal status and/or age are of importance for the development of genetically different forms of endometrial carcinomas, as judged from the DNA-distribution.
4 citations