The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article.

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Present and Future of Surface-Enhanced Raman Scattering

Damascene copper electroplating for on-chip interconnections, a process that we conceived and developed in the early 1990s, makes it possible to fill submicron trenches and vias with copper without creating a void or a seam and has thus proven superior to other technologies of copper deposition. We discuss here the relationship of additives in the plating bath to superfilling, the phenomenon that results in superconformal coverage, and we present a numerical model which accounts for the experimentally observed profile evolution of the plated metal.

Damascene copper electroplating for chip interconnections

http://www.dmphotonics.com/Femtosecond_Imaging/143.pdf

Principles of Two-Photon Excitation Microscopy and Its Applications to Neuroscience

Zero-dimensional, one-dimensional, two-dimensional and three-dimensional nanostructured materials for advanced electrochemical energy devices

Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by expression of defined embryonic factors. However, little is known of the molecular mechanisms underlying the reprogramming process. Here we explore somatic cell reprogramming by exploiting a secondary mouse embryonic fibroblast model that forms iPSCs with high efficiency upon inducible expression of Oct4, Klf4, c-Myc, and Sox2. Temporal analysis of gene expression revealed that reprogramming is a multistep process that is characterized by initiation, maturation, and stabilization phases. Functional analysis by systematic RNAi screening further uncovered a key role for BMP signaling and the induction of mesenchymal-to-epithelial transition (MET) during the initiation phase. We show that this is linked to BMP-dependent induction of miR-205 and the miR-200 family of microRNAs that are key regulators of MET. These studies thus define a multistep mechanism that incorporates a BMP-miRNA-MET axis during somatic cell reprogramming. PAPERCLIP:

Functional genomics reveals a bmp driven mesenchymal-to-epithelial transition in the initiation of somatic cell reprogramming (oral presentation)

Background
Natural biomaterials from bone-like minerals derived from avian eggshells have been considered as promising bone substitutes owing to their biodegradability, abundance, and lower price in comparison with synthetic biomaterials. However, cell adhesion to bulk biomaterials is poor and surface modifications are required to improve biomaterial-cell interaction. Three-dimensional (3D) nanostructures are preferred to act as growth support platforms for bone and stem cells. Although there have been several studies on generating nanoparticles from eggshells, no research has been reported on synthesizing 3D nanofibrous structures.

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Synthesis of three-dimensional calcium carbonate nanofibrous structure from eggshell using femtosecond laser ablation.

A laser-induced periodic surface structure (LIPSS) has attracted research interest for its promising potential in micromachining for microelectronics and microelectromechanical systems. A femtosecond laser-induced periodical surface structure was investigated for polished crystalline silicon. The observed structure is similar to the classical ripples that are characterized by long, nearly parallel lines extending over the entire irradiated area on the metal and silicon surface after continuous or pulsed laser irradiation. The spacing of the ripples nearly equals the irradiation wavelength. The depth of these ripples increases nonlinearly with the fluence of irradiation. The orientation of these periodic structures is perpendicular to the vector of the electric field of the laser beam. It seemed that the pattern formed by a femtosecond laser complies well with conventional models. Unlike the patterns formed by a continuous or nanosecond pulsed laser, however, the spacing of the ripple formed by femtosecond pulses is not influenced by the incident angle of the laser beam. The formula used to predict the ripple spacing in the conventional model does not apply to the femtosecond laser induced ripple structure. A plausible explanation to this phenomenon is proposed. The effect of the pulse repetition rate was studied and it was found that a femtosecond laser oscillator generates the same periodic structure as the amplified laser system.

https://iopscience.iop.org/article/10.1088/0960-1317/16/5/029/pdf

A femtosecond laser-induced periodical surface structure on crystalline silicon

Surface-enhanced Raman scattering (SERS)-based cancer diagnostics is an important analytical tool in early detection of cancer. Current work in SERS focuses on plasmonic nanomaterials that suffer from coagulation, selectivity, and adverse biocompatibility when used in vitro, limiting this research to stand-alone biomolecule sensing. Here we introduce a label-free, biocompatible, ZnO-based, 3D semiconductor quantum probe as a pathway for in vitro diagnosis of cancer. By reducing size of the probes to quantum scale, we observed a unique phenomenon of exponential increase in the SERS enhancement up to ~106 at nanomolar concentration. The quantum probes are decorated on a nano-dendrite platform functionalized for cell adhesion, proliferation, and label-free application. The quantum probes demonstrate discrimination of cancerous and non-cancerous cells along with biomolecular sensing of DNA, RNA, proteins and lipids in vitro. The limit of detection is up to a single-cell-level detection. Surface enhanced Raman scattering is a bio-analytical tool and the development and optimisation of probes is an active area of investigation. Here, the authors report on the development and testing of biocompatible semiconductor zinc oxide quantum probes on a platform for cell adhesion and analysis.

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Non plasmonic semiconductor quantum SERS probe as a pathway for in vitro cancer detection

Cancer stem cells (CSC) can be identified by modifications in their genomic DNA. Here, we report a concept of precisely shrinking an organic semiconductor surface-enhanced Raman scattering (SERS) probe to quantum size, for investigating the epigenetic profile of CSC. The probe is used for tag-free genomic DNA detection, an approach towards the advancement of single-molecule DNA detection. The sensor detected structural, molecular and gene expression aberrations of genomic DNA in femtomolar concentration simultaneously in a single test. In addition to pointing out the divergences in genomic DNA of cancerous and non-cancerous cells, the quantum scale organic semiconductor was able to trace the expression of two genes which are frequently used as CSC markers. The quantum scale organic semiconductor holds the potential to be a new tool for label-free, ultra-sensitive multiplexed genomic analysis. The low detection sensitivity of organic semiconductors has limited their use in biomedical surface-enhanced Raman scattering applications. Here, the authors use quantum scale organic semiconductors and show detection of genomic DNA methylation as well as gene expression.

/pdf/quantum-scale-organic-semiconductors-for-sers-detection-of-412d1lengm.pdf

Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression

This research work investigated drilling deep micro holes in semiconductor substrates with nanosecond laser ablation. The high pressure generated from the recoil of the ablated material and the expanding cloud of vapour and plasma leads to deformations in shape, including a barrel-shaped cross-section profile, bending of the hole channel and misalignment of the centre of the exit hole. It has been observed that deformations occur at all levels of pulse energy. To avoid the deformations and achieve smooth sidewalls, drilling with a multi-burst pulse train is proposed. First, a burst of pulses with low energy punches the substrate, producing a through hole smaller than the desired dimension. Then, a second burst of pulses with higher energy widens the exit hole and achieves holes with straight but tapered sidewalls. Since the through hole produced by the first burst provides an exit and expansion space for ablation plasma and vapour, the second burst will remove material without causing deformations. With this method we achieved 20 ?m holes with nearly straight sidewalls in a 250 ?m thick silicon substrate.

Bo Tan

Papers

Synthesis of three-dimensional calcium carbonate nanofibrous structure from eggshell using femtosecond laser ablation.

A femtosecond laser-induced periodical surface structure on crystalline silicon

Non plasmonic semiconductor quantum SERS probe as a pathway for in vitro cancer detection

Quantum scale organic semiconductors for SERS detection of DNA methylation and gene expression

Deep micro hole drilling in a silicon substrate using multi-bursts of nanosecond UV laser pulses