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Brian M. Turczyk
Researcher at Harvard University
Publications - 8
Citations - 1628
Brian M. Turczyk is an academic researcher from Harvard University. The author has contributed to research in topics: Mutation (genetic algorithm) & Gene expression. The author has an hindex of 6, co-authored 8 publications receiving 1285 citations. Previous affiliations of Brian M. Turczyk include Wyss Institute for Biologically Inspired Engineering.
Papers
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Journal ArticleDOI
Highly multiplexed subcellular RNA sequencing in situ.
Je-Hyuk Lee,Evan R. Daugharthy,Jonathan Scheiman,Reza Kalhor,Joyce L. Yang,Thomas C. Ferrante,Richard C. Terry,Sauveur S. F. Jeanty,Chao Li,Ryoji Amamoto,Derek T. Peters,Brian M. Turczyk,Adam H. Marblestone,Samuel A. Inverso,Amy Bernard,Prashant Mali,Xavier Rios,John Aach,George M. Church +18 more
TL;DR: FISSEQ is compatible with tissue sections and whole-mount embryos and reduces the limitations of optical resolution and noisy signals on single-molecule detection, and can be used to investigate cellular phenotype, gene regulation, and environment in situ.
Journal ArticleDOI
Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues
Je-Hyuk Lee,Evan R. Daugharthy,Jonathan Scheiman,Reza Kalhor,Thomas C. Ferrante,Richard C. Terry,Brian M. Turczyk,Joyce L. Yang,Hosuk Lee,John Aach,Kun Zhang,George M. Church +11 more
TL;DR: A protocol for genome-wide profiling of gene expression in situ in fixed cells and tissues, in which RNA is converted into cross-linked cDNA amplicons and sequenced manually on a confocal microscope, which enriches for context-specific transcripts over housekeeping and/or structural RNA.
Journal ArticleDOI
Forward Error Correction for DNA Data Storage
Meinolf Blawat,Klaus Gaedke,Ingo Hütter,Xiaoming Chen,Brian M. Turczyk,Samuel A. Inverso,Benjamin W. Pruitt,George M. Church +7 more
TL;DR: An efficient and robust forward error correction scheme adapted to the DNA channel is developed that is able to cope with all error types of today's DNA synthesis, amplification and sequencing processes, e.g. insertion, deletion, and swap errors.
Journal ArticleDOI
Precise Cas9 targeting enables genomic mutation prevention
Alejandro Chavez,Benjamin W. Pruitt,Marcelle Tuttle,Rebecca S. Shapiro,Rebecca S. Shapiro,Rebecca S. Shapiro,Ryan J Cecchi,Jordan Winston,Brian M. Turczyk,Michael Tung,James J. Collins,James J. Collins,James J. Collins,George M. Church +13 more
TL;DR: This study refine the specificity of the CRISPR-Cas9 system and presents a general framework for proactively preventing the occurrence of point mutations, including a generalized method of guide RNA “tuning” that enables Cas9 to discriminate between two target sites that differ by a single-nucleotide polymorphism.
Patent
Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
TL;DR: In this paper, a number of targeted nucleic acid FISSEQ library construction methods are presented, which can exhibit several benefits, such as enhanced sensitivity and shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species.