Bruno LePioufle

TL;DR: Osteoblastic cells could be successfully cultured inside the microdevices under dynamic conditions and their ALP activity was enhanced, promising for bone cell growth and differentiation as well as future tissue regeneration using larger 3D microfluidic microdevices.

TL;DR: Three-dimensional microfluidic systems in poly(dimethylsiloxane) (PDMS) were fabricated and used in conjunction with both custom-made and commercially available planar microelectrode arrays (pMEAs) to address the problem of in vitro culture of small neuronal networks with pre-defined topological features.

TL;DR: Two different micro-patterning methods for use in the growth guidance of dissociated primary culture neuron cells are compared: open chemically patterned growth substrates and enclosed micro-fluidic channels.

TL;DR: In this article, three-dimensional arrays of micro-wells for biological cell patterning and single-neuron guidance were made using inductively coupled plasma reactive ion etching (ICP-RIE) and SU-8 negative photoresist.

TL;DR: Several fabrication techniques for making three-dimensional arrays of micro-wells for biological cell patterning and single-neuron guidance are presented and methods for making complex 3d high-aspect-ratio structures in poly-dimethylsiloxane (PDMS) membrane are explored.