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Byung-Gee Kim

Researcher at Seoul National University

Publications -  410
Citations -  10826

Byung-Gee Kim is an academic researcher from Seoul National University. The author has contributed to research in topics: Streptomyces coelicolor & Gene. The author has an hindex of 52, co-authored 382 publications receiving 9479 citations. Previous affiliations of Byung-Gee Kim include New Generation University College & Chungnam National University.

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Journal Article

Identification of ω-aminotransferase from Caulobacter crescentus and site-directed mutagenesis to broaden substrate specificity.

TL;DR: The 3D model of the aminotransferase was constructed by homology modeling using a dialkylglycine decarboxylase PDB ID: 1DGE as a template and rationally redesigned to increase the activity for 3-amino- 3-phenylpropionic acid.
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Synthesis of enantiomerically pure trans-(1R,2R)- and cis-(1S,2R)-1-amino-2-indanol by lipase and omega-transaminase.

TL;DR: Syntheses of trans-(1R,2R) and cis-1-amino-2-indanol (AI) were accomplished by a series of enantioselective enzymatic reactions using lipase and transaminase (TA) and the kinetic resolution was performed with omega-TA.
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Microaffinity purification of proteins based on photolytic elution: toward an efficient microbead affinity chromatography on a chip.

TL;DR: The microaffinity purification allowed for a successful method for the identification of specific target proteins from a protein mixture and the feasibility of this system for use as a diagnosis chip was demonstrated.
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Engineering Transaminase for Stability Enhancement and Site-Specific Immobilization through Multiple Noncanonical Amino Acids Incorporation

TL;DR: The noncanonical amino acid incorporation technique is utilized to enhance the functional properties of ω‐transaminase (ω‐TA) by residue‐specific incorporation of (4R)‐fluoroproline [(4R]‐FP] and successfully immobilized onto chitosan or polystyrene (PS) beads with site‐specifically incorporated L‐3,4‐dihydroxyphenylalanine (DOPA) moiety.
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Increased in vivo immunological potency of HB-110, a novel therapeutic HBV DNA vaccine, by electroporation

TL;DR: It was shown that EP-based vaccination at 4-week-intervals elicited a cellular immune response which was about two-fold higher than the response elicited by conventional vaccination at 2-week intervals, which may provide a rationale to reduce the clinical dose and increase the interval between the doses in the multidose vaccination schedule.