B
Byung-Gee Kim
Researcher at Seoul National University
Publications - 410
Citations - 10826
Byung-Gee Kim is an academic researcher from Seoul National University. The author has contributed to research in topics: Streptomyces coelicolor & Gene. The author has an hindex of 52, co-authored 382 publications receiving 9479 citations. Previous affiliations of Byung-Gee Kim include New Generation University College & Chungnam National University.
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Journal ArticleDOI
Enzymatic synthesis of epothilone A glycosides.
Prakash Parajuli,Ramesh Prasad Pandey,Niranjan Koirala,Yeo Joon Yoon,Byung-Gee Kim,Jae Kyung Sohng +5 more
TL;DR: Epothilone A 7-O-β-D-glucoside was structurally elucidated by ultra-high performance liquid chromatography-photo diode array conjugated with high resolution quantitative time-of-flight-electrospray ionization mass spectroscopy (HR-QTOF ESI-MS/MS) supported by one-and two-dimensional nuclear magnetic resonance studies.
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Ecofriendly one-pot biosynthesis of indigo derivative dyes using CYP102G4 and PrnA halogenase
Seyun Namgung,Hyun A. Park,Joonwon Kim,Pyung-Gang Lee,Byung-Gee Kim,Yung-Hun Yang,Kwon-Young Choi +6 more
TL;DR: A one-pot producing strain which produced 7,7’-dichloroindigo from l -tryptophan using tryptophan-7-halogenase (PrnA) and CYP102G4 simultaneously was developed to overcome the disadvantages of uneconomical semi-synthesis through indole precursor feedstocks.
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How does dextran sulfate prevent heat induced aggregation of protein? The mechanism and its limitation as aggregation inhibitor.
TL;DR: It is found that the complex formation of the intermediate state ofDenatured BSA with dextran sulfate is a prerequisite to suppress the aggregation by preventing further oligomerization/aggregation process of denatured protein.
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Stereospecific synthesis of (R)-2-hydroxy carboxylic acids using recombinant E. coli BL21 overexpressing YiaE from Escherichia coli K12 and glucose dehydrogenase from Bacillus subtilis.
TL;DR: The yiaE gene from Escherichia coli K12 was functionally expressed in E. coliBL21 using an IPTG inducible pET expression system, and YiaE was purified to a specific activity of 18 U/mg, and the problem of NADPH recycle was effectively solved by using recombinantE.
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Multiplex Gene Disruption by Targeted Base Editing of Yarrowia lipolytica Genome Using Cytidine Deaminase Combined with the CRISPR/Cas9 System
TL;DR: Using this Target‐AID system with optimized expression levels of the base editor, single gene disruption and simultaneous double gene disruption are achieved with the efficiencies up to 94% and 31%, respectively, demonstrating this base editing system as a convenient genome editing tool in Y. lipolytica.