B
Byung-Gee Kim
Researcher at Seoul National University
Publications - 410
Citations - 10826
Byung-Gee Kim is an academic researcher from Seoul National University. The author has contributed to research in topics: Streptomyces coelicolor & Gene. The author has an hindex of 52, co-authored 382 publications receiving 9479 citations. Previous affiliations of Byung-Gee Kim include New Generation University College & Chungnam National University.
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"In-gel patch electrophoresis:" a new method for environmental DNA purification.
TL;DR: A rapid, simple, and efficient method for the purification of genomic DNA from environmental samples is described: a chromatography step directly embedded into an agarose gel electrophoresis, and it appeared that the purity grade was compatible with digestion by restriction enzymes and polymerase chain reaction (PCR) amplifications.
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NdgR, a Common Transcriptional Activator for Methionine and Leucine Biosynthesis in Streptomyces coelicolor
TL;DR: It is shown here that NdgR, a known transcriptional activator of isopropylmalate dehydratase in actinomycetes, may have other targets in the cell and proposed that N dgR induces cysteine-methionine biosynthesis, which would account for the abnormal growth phenotype of the ndGR knockout mutant.
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Effects of Sucrose, Phosphate, and Calcium Carbonate on the Production of Pikromycin from Streptomyces venezuelae.
TL;DR: To determine the most important nutritional factor and establish optimal culture conditions, medium optimization of pikromycin from Streptomyces venezuelae ATCC 15439 was studied with a statistical method, Plackett-Burman design and glucose, K2HPO4 and CaCO3 were determined to be the most significant factors.
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Enzymatic production of (R)-phenylacetylcarbinol by pyruvate decarboxylase from Zymomonas mobilis
Hyungdon Yun,Byung-Gee Kim +1 more
TL;DR: The synthesized (R)-phenylacetylcarbinol (PAC), a pharmaceutical intermediate for ephedrine and pseudoephedrine, from benzaldehyde and pyruvate using a recombinant pyruVate decarboxylase (PDC) from Zymomonas mobilis suggested that recombinant E. coli whole cell reactions are more efficient than crude enzyme extract or yeast-based reactions.
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Importance of spore mutants for fed-batch and continuous fermentation of Bacillus subtilis
TL;DR: Spore mutants can enhance subtilisin productivity and prolong subtILisin production time in fed‐batch culture as well as enable us to use very low dilution rates without losing productivity in continuous culture, thereby improving the conversion yield of the nitrogen source.