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/pdf/functional-nucleic-acid-sensors-k8tlieshyr.pdf

Functional Nucleic Acid Sensors

Immobilization is often the key to optimizing the operational performance of an enzyme in industrial processes, particularly for use in non-aqueous media. Different methods for the immobilization of enzymes are critically reviewed. The methods are divided into three main categories, viz. (i) binding to a prefabricated support (carrier), (ii) entrapment in organic or inorganic polymer matrices, and (iii) cross-linking of enzyme molecules. Emphasis is placed on relatively recent developments, such as the use of novel supports, e.g., mesoporous silicas, hydrogels, and smart polymers, novel entrapment methods and cross-linked enzyme aggregates (CLEAs).

http://largens.com/biochemlab/peroxidase2.pdf

Enzyme immobilization: The quest for optimum performance

Natural products have been a rich source of compounds for drug discovery. However, their use has diminished in the past two decades, in part because of technical barriers to screening natural products in high-throughput assays against molecular targets. Here, we review strategies for natural product screening that harness the recent technical advances that have reduced these barriers. We also assess the use of genomic and metabolomic approaches to augment traditional methods of studying natural products, and highlight recent examples of natural products in antimicrobial drug discovery and as inhibitors of protein-protein interactions. The growing appreciation of functional assays and phenotypic screens may further contribute to a revival of interest in natural products for drug discovery.

/pdf/the-re-emergence-of-natural-products-for-drug-discovery-in-1h0jebzmkp.pdf

The re-emergence of natural products for drug discovery in the genomics era

Utilization of polymers as biomaterials has greatly impacted the advancement of modern medicine. Specifically, polymeric biomaterials that are biodegradable provide the significant advantage of being able to be broken down and removed after they have served their function. Applications are wide ranging with degradable polymers being used clinically as surgical sutures and implants. In order to fit functional demand, materials with desired physical, chemical, biological, biomechanical and degradation properties must be selected. Fortunately, a wide range of natural and synthetic degradable polymers has been investigated for biomedical applications with novel materials constantly being developed to meet new challenges. This review summarizes the most recent advances in the field over the past 4 years, specifically highlighting new and interesting discoveries in tissue engineering and drug delivery applications.

/pdf/biomedical-applications-of-biodegradable-polymers-2db9gcyzxw.pdf

Biomedical Applications of Biodegradable Polymers

Innovative biotechnology for a green world and beyond.

Biochip fluorescence detection system with spatial and spectral separation

Orobol, 3'-hydroxy-genistein, suppresses the development and regrowth of cutaneous SCC.

Development of Quenching-qPCR (Q-Q) assay for measuring absolute intracellular cleavage efficiency of ribozyme

In this paper, proteins are purified and detected through bead-based bioassay on a silicon based micro biochip. Polystyrene micro beads pre-coated with specific capture molecules are packed into a microchip for a test. After binding target proteins, the proteins are re-separated from the beads using photolytic elution method. The photolytic elution method is enabled by introducing photo cleavable ligand on the beads. The purified target proteins are quantitatively detected using a fluorescence intensity measurement. The fluorescence measurements system is on-line coupled with the fabricated microchip. In fabrication, a silicon-on-glass structure is used so that a microchamber on microchip is transparent from the bottom to the top. Using this microchip, streptavidin is selectively purified and detected from low abundant protein mixture of human serum albumin, ovalbumin and streptavidin.

Byung-Gee Kim

Papers

Innovative biotechnology for a green world and beyond.

Biochip fluorescence detection system with spatial and spectral separation

Orobol, 3'-hydroxy-genistein, suppresses the development and regrowth of cutaneous SCC.

Development of Quenching-qPCR (Q-Q) assay for measuring absolute intracellular cleavage efficiency of ribozyme

Study on bead-based micro biochip and analytical system for protein detection