C
C. Loudes
Researcher at Collège de France
Publications - 13
Citations - 411
C. Loudes is an academic researcher from Collège de France. The author has contributed to research in topics: Polyunsaturated fatty acid & Chemically defined medium. The author has an hindex of 9, co-authored 13 publications receiving 405 citations.
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Journal ArticleDOI
Effect of Polyunsaturated Fatty Acids on Fetal Mouse Brain Cells in Culture in a Chemically Defined Medium
Jean-Marie Bourre,A. Faivre,Odile Dumont,A. Nouvelot,C. Loudes,Jack Puymirat,Andrée Tixier-Vidal +6 more
TL;DR: It is demonstrated that each of the four fatty acids studied was incorporated into phospholipids, and among several combinations of fatty acids, only 20:4 and 22:6, when added to the culture in a ratio of 2:1, restored a fatty acid profile similar to controls.
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Laminin promotes attachment and neurite elongation of fetal hypothalamic neurons grown in serum-free medium.
TL;DR: Laminin, as a coating or in solution, allows a rapid attachment of fetal hypothalamic cells in serum-free medium, and strikingly enhances the neurite network development, and in both culture conditions, their number decreases with time in vitro.
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Triiodothyronine enhances the morphological maturation of dopaminergic neurons from fetal mouse hypothalamus cultured in serum-free medium.
TL;DR: It is shown that hypothalamic dopaminergic neurons are responsive to triiodothyronine which acts as a maintenance or trophic factor having an effect on neurite extension and arborization.
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Effects of polyunsaturated fatty acids and hormones on synaptogenesis in serum-free medium cultures of mouse fetal hypothalamic cells.
TL;DR: The results show that mouse fetal hypothalamic cells cultured in chemically defined conditions display the same ability to respond to and to recover from potassium evoked depolarization as adult axon terminals, and offer a suitable model for analysis of the mechanisms involved in membrane traffic in central neurons.
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Release of immunoreactive TRH in serum-free cultures of mouse hypothalamic cells
TL;DR: Serum-free cultures of mouse hypothalamic cells were used as a model for studying TRH (thyroliberin) secretion in vitro and depolarization of the cells with 60 mM K+ results in a calcium-dependent release of immunoreactive TRH.