C Olivier

TL;DR: A nonisotopic PCR assay in which sequences from the beta-globin gene and from the HIV-1 vpu-env region were coamplified with biotinylated and fluorescein-labeled primers, respectively allowed for the evaluation of the quality of specimens for PCR concurrently with the detection of the presence of viral template sequences.

TL;DR: This study demonstrates that commercialized nonisotopic PCR assays reach adequate levels of sensitivity and specificity for diagnosis of HIV-1 infection and could be considered in clinical situations in which serology is not helpful.

TL;DR: Prolonged infection with HIV-1 without seroconversion was not found in the population of patients at very high risk for HIV- 1 infection, and two of the three PCR-positive seronegative patients had symptoms suggestive of acute retroviral disease.

TL;DR: A convenient assay combining solution hybridization and enzyme immunoassay for DNA-RNA hybrids (polymerase chain reaction-enzyme immunoASSay [PCR-EIA]) was developed to detect human immunodeficiency virus type 1 (HIV-1) provirus amplified by the PCR and was compared with oligomer hybridization with 32P-labeled SK19.