Journal ArticleDOI
Discordance between primer pairs in the polymerase chain reaction for detection of human immunodeficiency virus type 1: a role for taq polymerase inhibitors.
François Coutlée,Pierre Saint-Antoine,C Olivier,A Vessous-Elbaz,Hélène Voyer,Fouad Berrada,P Bégin,L Giroux,Raphael P. Viscidi +8 more
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This article is published in The Journal of Infectious Diseases.The article was published on 1991-10-01. It has received 30 citations till now. The article focuses on the topics: Taq polymerase & Nested polymerase chain reaction.read more
Citations
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Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy.
TL;DR: A prospective 'blinded' comparison of diagnosis by conventional light microscopy and enzymatic amplification of the circumsporozoite gene extracted from dried spotted blood samples found P. falciparum and P. vivax infections to be mixed by the PCR/DNA probe systems.
Journal ArticleDOI
Detection of plasmodium falciparum by polymerase chain reaction in a field study
TL;DR: The ease of collection and transport of filter-paper specimens combined with the sensitive and specific detection of allelic genes of P. vivax by PCR suggests that this method may prove to be a valuable tool for epidemiologic and heterogeneity studies of the parasite.
Journal ArticleDOI
Performance of the Amplicor human immunodeficiency virus type 1 PCR and analysis of specimens with false-negative results.
TL;DR: Over a 4-year period, the Roche Amplicor kit was used in a United Kingdom reference laboratory for the detection or confirmation of human immunodeficiency virus (HIV) type 1 infection, particularly in infants born to HIV-infected mothers.
Journal ArticleDOI
Circumsporozoite genotyping of global isolates of Plasmodium vivax from dried blood specimens.
Kevin C. Kain,A. E. Brown,H K Webster,Robert A. Wirtz,Jay S. Keystone,Mario H. Rodriguez,J Kinahan,M Rowland,David E. Lanar +8 more
TL;DR: It is concluded that use of samples of whole blood on filter paper is a practical and sensitive method for determining the genotypes of large numbers of malaria isolates collected in field settings.
Journal ArticleDOI
Improved detection of JC virus DNA in cerebrospinal fluid for diagnosis of AIDS-related progressive multifocal leukoencephalopathy.
A. De Luca,Antonella Cingolani,Angela Linzalone,Adriana Ammassari,Rita Murri,Maria Letizia Giancola,G Maiuro,Andrea Antinori +7 more
TL;DR: Several methods to increase the sensitivity of JC virus (JCV) DNA detection in cerebrospinal fluid (CSF) for a noninvasive diagnosis of AIDS-related progressive multifocal leukoencephalopathy (PML) were investigated.
References
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Amplification of HIV-1 Provirus from Cerebrospinal Fluid and Its Correlation with Neurologic Disease
Sunil Shaunak,Robert E. Albright,Mary E. Klotman,Stanley C. Henry,John Bartlett,John D. Hamilton +5 more
TL;DR: PCR of CSF nucleated cells from HIV-positive patients provides early, rapid, direct evidence of neurologic involvement, and detects HIV-1 proviral sequences (gag and env) in nucleate cells from the cerebrospinal fluid.