Carl W. Fuller

TL;DR: Fluorescent dye-labeled DNA primers have been developed that exploit fluorescence energy transfer (ET) to optimize the absorption and emission properties of the label and should be generally useful in the development of other multiplex DNA sequencing and analysis methods.

TL;DR: A single-molecule nanopore-based sequencing by synthesis approach that accurately distinguishes four DNA bases by electronically detecting and differentiating four different polymer tags attached to the terminal phosphate of the nucleotides during their incorporation into a growing DNA strand in the polymerase reaction is demonstrated.

TL;DR: The increased fluorescence intensity of the ET primers and the substantially similar mobilities of the DNA fragments generated with the four ET primer allow four-color DNA sequencing on a capillary electrophoresis DNA sequencer using a single laser line at 488 nm for excitation and without applying mobility shift adjustments.

TL;DR: The design and characterization of a DNA polymerase–nanopore protein construct on an integrated chip incorporates all four tagged nucleotides and distinguishes single–tagged-nucleotide addition in real time, and is observed during polymerase-catalyzed DNA synthesis.

TL;DR: In this paper, a disdosure of a tagged nudeotide is described, comprising a polyphosphate moiety having a terminal phosphate and a tag covalently coupled to the terminal phosphate of the nudeotide by a triazole, a 1,2-diazine, a disulfide, a secondary amine, hydrazone, a thio-acetamide, or a maleimide-thioadduct.