Showing papers in "Analytical Biochemistry in 1995"

TL;DR: Using the optimized reaction conditions it has been shown that molar labeling efficiencies are high and essentially independent of the amount of glycans and glycoprotein-derived glycan libraries and also allows relative molar quantitation of individual glycans in a pool.

TL;DR: S1-PFGE avoids the difficulties of plasmid isolation, eliminates the preparation of probes, and does not require knowledge of restriction enzyme cleavage sites and can be used to screen for megaplasmids in many strains simultaneously.

TL;DR: Multiwavelength near-infrared attenuation spectra on human forearm muscle, the adult rat head, and newborn piglet head are collected to compare the changes in chromophore concentration derived from these data using published algorithms from four groups, finding differences between the results from the algorithms on each data set.

TL;DR: 27, 24, and 7 alpha-hydroxycholesterol were the most abundant cholesterol oxidation products in human plasma and the other oxysterols determined were present in concentrations lower than 30 ng/ml.

TL;DR: A calculation procedure of general validity has been developed to unravel the effect of rapid exchange between the various species in equilibrium as a function of pD of the solution to obtain basicity constants of spermine with respect to the D+ cation at 40 degrees C.

TL;DR: A SOD microassay based on spectrophotometric assessment of O2.- mediated nitro blue tetrazolium reduction by an aerobic mixture of NADH and phenazine methosulfate, which produces superoxide chemically at nonacidic pH is developed.

TL;DR: Global analysis, in combination with numerical integration, provided a stringent test of the assumed model and stringency suggests that its application to experimental systems will require high-quality biosensor data.

TL;DR: A critical review of numerous published variations of the key steps in the RACE method is presented and a detailed, effective protocol based on RNA ligase-mediated RACE/reverse ligation-mediated PCR is presented, as well as a demonstration of its utility.

TL;DR: An assay for the simultaneous measurement of nitrite and nitrate, products of nitric oxide metabolism, is described and it is found that the NADP+ formed during pretreatment interfered with the Griess reaction when NADPH was used at concentrations necessary to drive the NR reaction.

TL;DR: Sets of parameters governing the rules were determined under various mutagenic conditions including the addition of MnCl2 and validity of the rules was assessed in several mutagenesis experiments showing that a wide range of substitution frequencies including AT-->GC and GC-->AT transitions as well as AT-->TA transversions can be obtained at will.

TL;DR: The use of multispot sensing thus extends direct interaction analysis to include low-molecular-weight analytes and low-affinity interactions.

TL;DR: This method has proven to be highly sensitive, requiring only 1 pmol for reliable detection of neutral and amino sugars from both oligosaccharide and glycoprotein samples, and quantitation of sialic acids works equally well with both free oligOSaccharides and glyCoprotein samples.

TL;DR: This work describes a simple solution to limit degradation of the DNA template and suggests that the DNA fragmentation which occurs limits the sensitivity of the bisulfite method.

TL;DR: A rapid nonradioactive colorimetric assay that utilizes a beta-galactosidase gene fused to five copies of the cyclic AMP response element (CRE) to detect the activation of CRE-binding protein that results from an increase in intracellular cAMP or calcium is developed.

TL;DR: A new method for measurement of glutathione and other biological thiols utilizing reverse-phase high-performance liquid chromatography to separate and quantify these derivatives is described, offering some advantages over the currently accepted techniques, including specificity, speed, sensitivity, and ease of use.

TL;DR: HBMs are shown to be stable in flow and to block nonspecific adsorption of proteins to the alkanethiol/gold surface and provide a well-defined surface that will permit less ambiguous modeling of these important biological interactions.

TL;DR: A new type of optical biosensor system is proposed and its application to detection of antigen-antibody complexes on a silicon substrate is demonstrated and Visualization of the thickness distributions of thin layers on a surface is achieved by using imaging ellipsometry.

TL;DR: The reduction and alkylation step was introduced since experiments with lysozyme and ribonuclease A showed that extremely complex peptide patterns were obtained if no precautions were taken to suppress disulfide bond formation during in-gel digestion of proteins.

TL;DR: A comparative study of the ratiometric and fluorescence lifetime techniques using carboxy SNAFL-1 for pH determination demonstrates that thefluorescence lifetime imaging technique is more convenient than theRatiometric method for pH determined.

TL;DR: A concept for the determination of single-point mutations in DNA samples is described, which was employed to analyze multistep solid-phase gene assembly and the performance of different enzymes routinely used for the synthesis and manipulation of DNA.

TL;DR: The proposed method is based on the use of O-(2, 3, 4, 5, 6-pentafluorobenzyl) hydroxylamine hydrochloride to form the PFB-oxime-TMS derivatives, which allows the measurement of physiological aldehyde levels in biological samples.

TL;DR: A direct colorimetric assay for copper in serum and biological samples using 2,2'-bicinchoninic acid (BCA), a common reagent in most laboratories, and the results correlate strongly with atomic absorption spectrophotometry.

TL;DR: A selective and reproducible high-performance liquid chromatographic method for the quantification of ascorbic acid and dehydroascorbic acid in plasma is developed and validated and meets the requirements of a reliable routine analysis.

TL;DR: A three-dimensional (3-D) sugar-mapping technique for pyridylaminated (PA) neutral and sialyl oligosaccharides as a powerful structural characterization of N-linked oligosACcharides using only picomoles of samples is proposed.

TL;DR: This new scanning calorimeter operates differentially, is equipped with a pair of gold capillary cells and semiconductor sensors, and is able to scan up and down in temperature at user-selected rates.