Cédric Atmanene

TL;DR: A description of the general approach and an overview of the possible characterization of ligand-binding properties are provided, as well as how native MS supports structure- and fragment-based drug research is discussed.

TL;DR: Investigations focused on mAb:Ag complexes revealed that, similarly to murine mAb, humanized mAb 6F4 binds selectively up to four antigen molecules with a similar affinity, confirming in this way the reliability of the humanization process.

TL;DR: The tetrameric oligomerization state of TrmI is confirmed, and it is proposed that the conserved residues that form the catalytic cavity are essential for fashioning an optimized shape of the catalysttic pocket.

TL;DR: While the combined results of these screening methods retrieve 10 of the 11 crystal structures originally predicted by the biochemical assay, the mutual overlap of individual hit lists is surprisingly low, highlighting that each technique operates on different biophysical principles and conditions.

TL;DR: Noncovalent mass spectrometry was used to confirm the dimeric oligomerization state of Tgt in solution and evidence the binding stoichiometry of the complex formed between Tgt and its full-length substrate tRNA and the effect of inhibitor binding was investigated, thus providing thebinding stoichiometries of TGT:inhibitor complexes and showing competitive interactions in the presence of tRNA.