Showing papers by "Cedric S. Raine published in 1979"
TL;DR: The results indicate that oligodendrocyte staining is not specific for MS, is not due to specific antibody, and is probably the result of nonspecific binding to Fc receptors.
Abstract: The immunofluorescent staining properties of 65 serum samples from 54 patients with multiple sclerosis (MS), 63 samples from 55 patients with other neurological diseases (OND), and sera from 14 healthy normal individuals were examined on frozen sections of bovine and human brain. When tested on bovine brain sections, positive oligoden drocyte staining was present in 63% of MS sera, 43% of OND sera, and 29% of normal sera. The percentages were lower with human brain tissue. Astrocyte and myelin staining was common. F(ab')2 fragments purified from selected positive and negative MS and control sera gave no staining, though IgG fractions from the same sera prior to pepsin digestion gave positive staining. When tested against antihuman IgM and IgA conjugates, the same positive sera and their IgG-depleted globulin fractions gave minimal or no staining. These results indicate that oligodendrocyte staining is not specific for MS, is not due to specific antibody, and is probably the result of nonspecific binding to Fc receptors.
65 citations
TL;DR: The results indicate that chronic relapsing EAE can be successfully suppressed with MBP in IFA, that this suppression is permanent and that the immunologic findings presented correlate well with the clinical and pathologic facets of the disease.
39 citations
TL;DR: Circulating lymphocyte populations were examined in 85 patients with multiple sclerosis, 26 of whom showed exacerbations, and patients with other neurological diseases (OND); 14 patients suffering from psychiatric disorders; and 2 normal subjects, and MBP‐reactive early T‐cells were more frequently increased in MS than OND, and while this might be indicative of increased sensitization against myelin antigens, it was found not to be an MS‐specific phenomenon.
Abstract: Circulating lymphocyte populations were examined in 85 patients with multiple sclerosis (MS), 26 of whom showed exacerbations; 48 patients with other neurological diseases (OND); 14 patients suffering from psychiatric disorders; and 2 normal subjects. The study involved the assay of early (active, high-affinity rosetting) T-cells, myelin basic protein (MBP)-reactive early T-cells, late (total, 24-hour rosetting) T-cell levels were significantly lower in MS (p less than 0.01) than in OND subjects. Exacerbations in MS were usually accompanied by further decreases in early T-cells. The lower levels of early T-cells in MS and their fluctuations are believed to reflect disease activity. MBP-reactive early T-cells were more frequently increased in MS (75% of cases) than OND (50%), and while this might be indicative of increased sensitization against myelin antigens, it was found not to be an MS-specific phenomenon.
31 citations
TL;DR: MBP-reactive early T cells were determined simultaneously in the circulation and in inflammatory cells from the meninges of guinea pigs with acute EAE to indicate that the majority of early T cell in meningeal infiltrates had been previously activated by MBP in vivo.
20 citations
TL;DR: An ununual case of concurrent MS and anaplastic astrocytoma is presented in this paper, where a left side thalamotomy was performed for relief of severe intention tremor at age 28 and at age 32 she received immunosuppressive therapy for 1 year.
Abstract: An ununsual case of concurrent MS and anaplastic astrocytoma is presented. MS was diagnosed in a female patient at the age of 22 years. A left side thalamotomy was performed for relief of severe intention tremor at age 28 and at age 32 she received immunosuppressive therapy for 1 year. At the age of 36 after a severe exacerbation of her symptoms a left side fronto-temporal tumor was diagnosed and a subtotal neurosurgical extirpation was performed. Histopathologically, the tumor was an anaplastic astrocytoma, which was further substantiated by electron microscopy and establishment of a permanent cell line in vitro. The cultured tumor cells were negative for measles virus by immunofluorescence. The relationship between the reactive astrocytes in MS plaques and astrocytic neoplasia is discussed.
10 citations
TL;DR: A heterogeneous population of virions is generated by measles virus-infected cells and these particles are partially separable by sucrose density centrifugation into three peaks, each of which contains infectious particles.
Abstract: A heterogeneous population of virions is generated by measles virus-infected cells. These particles are partially separable by sucrose density centrifugation into three peaks. Each population is stable and contains infectious particles. The particles of all three populations contain at least six polypeptide species that differ between particle populations only in quantity. All three populations contain a 50S RNA species, and the heaviest density peak also contains an additional species of 43S RNA. The difference between these results and previous studies with measles virions will be discussed.
9 citations
TL;DR: In this article, the authors reported that following repeated short-interval blood sampling by cardiac puncture for the estimation of lymphocyte populations, some adult Strain 13 guinea pigs sensitised for acute EAE unexpectedly survived and developed chronic EAE, while a group of juveniles sensitized for chronic encephalomyelitis and bled under the same conditions, developed a more severe, acute form of EAE.
Abstract: Previous studies have shown that Strain 13 guinea pigs sensitised for experimental allergic encephalomyelitis (EAE) as adults usually develop an acute, fatal form of disease while animals inoculated as juveniles usually display a chronic relapsing form. The present study reports that following repeated short-interval blood sampling by cardiac puncture for the estimation of lymphocyte populations, some adult Strain 13 guinea pigs sensitised for acute EAE unexpectedly survived and developed chronic EAE, while a group of juveniles sensitised for chronic EAE and bled under the same conditions, developed a more severe, acute form of EAE. It is suggested that this reversal of disease course was related to the depletion of circulating factors.
8 citations
TL;DR: Tissue prisms prepared by choping whole mouse brain maintained respiratory capacity and ultrastructural integrity of 3 h in vitro and suggest the prism system is simpler than that of organotypic cultures and may be useful in the study of myelinotoxic factors.
4 citations