Charles D. Yeh

TL;DR: DISCOVER-Seq as mentioned in this paper is a universally applicable approach for unbiased off-target identification that leverages the recruitment of DNA repair factors in cells and organisms, tracking the precise recruitment of MRE11 uncovers the molecular nature of Cas activity in cells with single base resolution.

TL;DR: This Review discusses the DNA repair pathways that underlie genome editing and recent improvements and strategies to yield desired genomic alterations in cells and organisms.

TL;DR: A powerful, sensitive assay for the unbiased identification of off- target sites that is applicable to multiple types of Cas nucleases and provides an unprecedented view of events that precede repair of the affected sites, paving the way for real-time off-target discovery during therapeutic gene editing.

TL;DR: A method to detect off-targets of CRISPR–Cas genome editing based on ChIP-seq analysis of MRE11 recruitment to DSBs, and subsequent bioinformatics analysis of sequencing data using the BLENDER pipeline.

TL;DR: A potential role of FBXO2-dependent glioma-microenvironment interactions to promote tumor growth is demonstrated and the published datasets provide a valuable resource for further studies.