P
Pinar Akcakaya
Researcher at Karolinska University Hospital
Publications - 25
Citations - 1550
Pinar Akcakaya is an academic researcher from Karolinska University Hospital. The author has contributed to research in topics: Genome editing & GiST. The author has an hindex of 14, co-authored 23 publications receiving 1214 citations. Previous affiliations of Pinar Akcakaya include Loyola University Medical Center & AstraZeneca.
Papers
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Journal ArticleDOI
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq.
Beeke Wienert,Beeke Wienert,Stacia K. Wyman,Christopher D. Richardson,Charles D. Yeh,Pinar Akcakaya,Michelle J. Porritt,Michaela Morlock,Jonathan T. Vu,Katelynn R. Kazane,Hannah L. Watry,Hannah L. Watry,Luke M. Judge,Luke M. Judge,Bruce R. Conklin,Bruce R. Conklin,Marcello Maresca,Jacob E. Corn +17 more
TL;DR: DISCOVER-Seq as mentioned in this paper is a universally applicable approach for unbiased off-target identification that leverages the recruitment of DNA repair factors in cells and organisms, tracking the precise recruitment of MRE11 uncovers the molecular nature of Cas activity in cells with single base resolution.
Journal ArticleDOI
In vivo CRISPR editing with no detectable genome-wide off-target mutations
Pinar Akcakaya,Maggie L. Bobbin,Jimmy A. Guo,Jose Malagon-Lopez,Kendell Clement,Sara P. Garcia,Mick D. Fellows,Michelle J. Porritt,Mike Firth,Alba Carreras,Alba Carreras,Tania Baccega,Frank Seeliger,Mikael Bjursell,Shengdar Q. Tsai,Nhu T. Nguyen,Roberto Nitsch,Lorenz M. Mayr,Lorenz M. Mayr,Luca Pinello,Mohammad Bohlooly-Y,Martin J. Aryee,Marcello Maresca,J. Keith Joung +23 more
TL;DR: VIVO provides a general strategy for defining and quantifying the off-target effects of gene-editing nucleases in whole organisms, thereby providing a blueprint to foster the development of therapeutic strategies that use in vivo gene editing.
Journal ArticleDOI
The role of microRNA deregulation in the pathogenesis of adrenocortical carcinoma
Deniz M. Ozata,Stefano Caramuta,David Velázquez-Fernández,Pinar Akcakaya,Hong Xie,Anders Höög,Jan Zedenius,Martin Bäckdahl,Catharina Larsson,Weng-Onn Lui +9 more
TL;DR: The functional role of four miRNAs in the pathogenesis of ACC cells is elucidated, and the potential involvement of the pro-apoptotic factor PUMA (a miR-483-3p target) in adrenocortical tumors is demonstrated.
The role of microRNA deregulation in the pathogenesis of
Deniz M. Ozata,Stefano Caramuta,David Velázquez-Fernández,Pinar Akcakaya,Hong Xie,Anders Höög,Jan Zedenius,Martin Bäckdahl,Catharina Larsson +8 more
Journal ArticleDOI
miR-185 and miR-133b deregulation is associated with overall survival and metastasis in colorectal cancer.
Pinar Akcakaya,Susanne Ekelund,Iryna Kolosenko,Stefano Caramuta,Deniz M. Ozata,Hong Xie,Ulrik Lindforss,Hans Olivecrona,Weng-Onn Lui +8 more
TL;DR: The SYBR-green quantitative PCR-based array approach was applied to screen for differentially expressed miRNAs between patients with short and long survival and found that high expression of miR-185 and low expression ofMiR-133b were correlated with poor survival and metastasis in colorectal cancer.