Christine Surka

TL;DR: An enhanced CLIP (eCLIP) protocol is developed that decreases requisite amplification by ∼1,000-fold, decreasing discarded PCR duplicate reads by ∼60% while maintaining single-nucleotide binding resolution, and improves specificity in the discovery of authentic binding sites.

TL;DR: In this article, the authors investigated the localization mechanisms of the Xist lncRNA during X-chromosome inactivation (XCI), a paradigm of lncRN-mediated chromatin regulation.

TL;DR: A method to purify a lncRNA from cells and identify proteins interacting with it directly using quantitative mass spectrometry is developed and it is shown that SHARP, which interacts with the SMRT co-repressor that activates HDAC3, is not only essential for silencing, but is also required for the exclusion of RNA polymerase II from the inactive X.

TL;DR: The data suggest a model for how Xist can integrate its two functions—localization to DNA and silencing of gene expression—to coat the entire X chromosome.

TL;DR: In this article, a method based on RNA antisense purification was developed to systematically map RNA-RNA interactions (RAP-RNA) and applied it to investigate two ncRNAs implicated in RNA processing.