Cindy Lou Chepanoske

TL;DR: It is demonstrated that high-throughput screening for protein interactions combined with genetic validation in a model organism is a powerful approach for identifying novel candidate modifiers of polyglutamine toxicity.

TL;DR: A drug‐target system consisting of ampicillin‐ and penicillin‐binding proteins (PBPs) to evaluate and compare different amino‐reactive resins for the immobilization of the affinity compound and mass spectrometric methods to identify proteins from drug affinity pull‐down assays is investigated.

TL;DR: A trend is observed in a simple relationship, derived from standard database search outputs, which can be useful in assessing the quality of a MS/MS-based protein identification, and the ratio of the protein score and number of non-redundant peptides, or average peptide score (APS), can facilitate initial filtering of database search results.

TL;DR: In this paper, the authors proposed methods of screening for binding partners, especially binding partners essential for the biological activity of erastin (e.g. VDACs).

TL;DR: A multi-step process for the discovery of protein–protein interactions is described and it is shown that process stages are interdependent and can influence, either positively or negatively, subsequent steps.