Cliona Boyle

TL;DR: The results indicate that the AGE/ALE inhibitor PM protected against a range of pathological changes in the diabetic retina and may be useful for treating diabetic retinopathy.

TL;DR: In this article, the authors used a LightCycler rapid thermal cycler (Roche) to detect SYBR Green I fluorescence and applied this method in an experimental model to quantify a wide range of known VEGF template concentrations, and then used 20 existing primer pairs to quantify their cognate mRNAs.

TL;DR: The sequence-independent detection of DNA with SYBR Green I means that it can be used to quantify the amplification of any cDNA using gene-specific primers and is ideally suited for researchers in vision science wishing to quantify mRNAs from many different genes because it does not require investment in gene- specific hybridization probes.

TL;DR: This work reports the development of a reporter system based on fluorescence dilution that enables direct quantification of the replication dynamics of Salmonella enterica serovar Typhimurium in murine macrophages at both the population and single-cell level and finds that, upon entry into macrophage, many bacteria do not replicate, but appear to enter a dormant-like state.

TL;DR: Ubiquitin-modified proteins accumulated in macrophages infected with Salmonella sseL mutant strains but to a lesser extent when infected with bacteria expressing active protein, demonstrating that SseL functions as a deubiquit inase in vivo.