Corrado Viotti

TL;DR: Examination of secretory and endocytotic trafficking in Arabidopsis provides evidence that both endocytic and secretory cargo pass through the trans-Golgi network/early endosome (TGN/EE) and demonstrates that cargo in late endosomes/multivesicular bodies is destined for vacuolar degradation.

TL;DR: The results demonstrate that a selective process such as ubiquitylation can lead to the degradation of a key regulatory protein such as AGO1 by a degradation process generally believed to be unspecific.

TL;DR: Evidence is presented that post-TGN transport toward lytic vacuoles occurs independently of clathrin and that MVBs/LEs are derived from the TGN/EE through maturation, indicating that MVB originate from theT GN/EE in a process that requires the action of ESCRT for the formation of intraluminal vesicles and annexins for the final step of releasing MVBs as a transport carrier to the vacuole.

TL;DR: This work uses genetic and pharmacological interference in combination with live-cell imaging, three-dimensional reconstruction, and electron microscopy to monitor trafficking to the tonoplast of the two proton pumps, V-ATPase and V-PPase, providing strong evidence for a Golgi-independent route of vacuolar biogenesis in plant cells.

TL;DR: Using computational modeling, it is shown that the simplest scenario to explain pavement cell shapes within an epidermis under tension must involve mechanical wall heterogeneities across and along the anticlinal pavement cell walls between adjacent cells.